Immunofluorescence analysis of S100 was performed using 70% confluent log phase A-375 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with S100 Rabbit Polyclonal Antibody (PA516257) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic and nuclear localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Bovine, Chicken, Human, Marsupial, Mouse, Rat|
|Published species reactivity||Rat, Sheep, Human, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||S100 protein purified from cow brain|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:200|
|Western Blot (WB)||1-3 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-16257 targets S100 Protein in ICC and IHC (P) applications and shows reactivity with Bovine, Chicken, Human, Marsupial, mouse, and Rat samples.
The PA5-16257 immunogen is s100 protein purified from cow brain.
S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. Antibody to S100 stains Schwannomas, ependymomas, astrogliomas, almost all benign and malignant melanomas and their metastases. S100 protein is also expressed in the antigen presenting cells such as the Langerhans cells in skin and interdigitating reticulum cells in the paracortex of lymph nodes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Papillary-cystic pattern is characteristic in mammary analogue secretory carcinomas but is rarely observed in acinic cell carcinomas of the salivary gland.
PA5-16257 was used in immunohistochemistry - paraffin section to subtype mammary analogue secretory carcinoma and acinic cell carcinoma histologically
|Hsieh MS,Chou YH,Yeh SJ,Chang YL||Virchows Archiv : an international journal of pathology (467:145)||2015|
Expression of cellular components in granulomatous inflammatory response in Piaractus mesopotamicus model.
PA5-16257 was used in immunohistochemistry - paraffin section to study a Piaractus mesopotamicus model and expression of cellular components in granulomatous inflammatory response
|Manrique WG,Claudiano Gda S,de Castro MP,Petrillo TR,Pereira Figueiredo MA,Belo MA,Berdeal MI,de Moraes JE,de Moraes FR||PloS one (10:null)||2015|
Intravenous AAV9 efficiently transduces myenteric neurons in neonate and juvenile mice.
PA5-16257 was used in immunohistochemistry - paraffin section to determine the enteric nervous system transduction following systemic AAV9 administration
|Gombash SE,Cowley CJ,Fitzgerald JA,Hall JC,Mueller C,Christofi FL,Foust KD||Frontiers in molecular neuroscience (7:null)||2014|
Cortical hypoplasia and ventriculomegaly of p73-deficient mice: Developmental and adult analysis.
PA5-16257 was used in immunohistochemistry to study the pathophysiology of two main phenotypic variants of murine brain p73 deficiency
|Medina-Bolívar C,González-Arnay E,Talos F,González-Gómez M,Moll UM,Meyer G||The Journal of comparative neurology (522:2663)||2014|
MT1-MMP modulates melanoma cell dissemination and metastasis through activation of MMP2 and RAC1.
PA5-16257 was used in immunohistochemistry to study the role of MMP-2 and RAC1 activation in the mechanism by which MT1-MMP promotes melanoma cell motility and metastasis
|Shaverdashvili K,Wong P,Ma J,Zhang K,Osman I,Bedogni B||Pigment cell and melanoma research (27:287)||2014|
Expression of insulin-like growth factor binding proteins during mouse cochlear development.
PA5-16257 was used in immunohistochemistry to study the expression of IGFBP family members during development of the murine cochlear
|Okano T,Kelley MW||Developmental dynamics : an official publication of the American Association of Anatomists (242:1210)||2013|
Low-copy piggyBac transposon mutagenesis in mice identifies genes driving melanoma.
PA5-16257 was used in immunohistochemistry to identify key melonoma genes using a piggyBac transposon mutagenesis approach in a murine model
|Ni TK,Landrette SF,Bjornson RD,Bosenberg MW,Xu T||Proceedings of the National Academy of Sciences of the United States of America (110:E3640)||2013|
Cutaneous innervation of the human face as assessed by skin biopsy.
PA5-16257 was used in immunohistochemistry to use skin biopsy to study human facial cutaneous innervation
|Nolano M,Provitera V,Caporaso G,Stancanelli A,Leandri M,Biasiotta A,Cruccu G,Santoro L,Truini A||Journal of anatomy (222:161)||2013|
Veil nerve-sparing technique and postoperative urinary continence in open antegrade radical prostatectomy.
PA5-16257 was used in immunohistochemistry to describe a novel technique for open antegrade radical prostatectomy
|Shigemura K,Yamanaka N,Yamashita M||Urologia internationalis (89:283)||2012|
SPARC-like 1 (SC1) is a diversely expressed and developmentally regulated matricellular protein that does not compensate for the absence of SPARC in the CNS.
PA5-16257 was used in immunohistochemistry to study the expression and role of SPARC-like 1 in the developing and adult nervous system
|Lloyd-Burton S,Roskams AJ||The Journal of comparative neurology (520:2575)||2012|
A case of xanthoma in a Saanen goat.
PA5-16257 was used in immunohistochemistry to report on a case of a Saanen goat with xanthoma
|Ozmen O,Haligur M||Veterinary dermatology (23:150)||2012|
A novel electrospun nerve conduit enhanced by carbon nanotubes for peripheral nerve regeneration.
PA5-16257 was used in immunocytochemistry to develop a novel nerve conduit incorporating carbon nanotudes for use in the regeneration of peripheral nerves
|Yu W,Jiang X,Cai M,Zhao W,Ye D,Zhou Y,Zhu C,Zhang X,Lu X,Zhang Z||Nanotechnology (25:null)||2014|
A microscale neuron and Schwann cell coculture model for increasing detection sensitivity of botulinum neurotoxin type A.
PA5-16257 was used in immunocytochemistry to develop a novel bioasay for the highly sensitive detection of botulinum toxin type A based on the co-culture of neurons and Schwann cells on a microscale
|Hong WS,Young EW,Tepp WH,Johnson EA,Beebe DJ||Toxicological sciences : an official journal of the Society of Toxicology (134:64)||2013|