Immunofluorescent analysis of S100 alpha (red) in HEK293T cells. Cells fixed with 4% formaldehyde were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were probed with a S100 alpha polyclonal antibody (Product # PA1-932) at a dilution of 1:100 overnight at 4°C in 1X PBS containing 1% BSA and 0.3% Triton X-100, washed with 1X PBS, and incubated with a fluorophore-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:200 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI. Images were taken on a Leica DM1000 microscope at 40X magnification. Data courtesy of the Innovators Program.
|Tested species reactivity||Bovine, Human, Mouse, Pig, Rat|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Purified S100-alpha protein from human pectoral muscle cells.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 15mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1 ug/ml|
|Immunoprecipitation (IP)||5 ug/ml|
|Western Blot (WB)||0.1 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-932 detects S100-alpha protein in bovine, human, mouse, porcine, and rat samples. This antibody has shown no cross-reactivity with the S100-beta protein.
PA1-932 has successfully been used in Western blot, ELISA, immunofluorescence, immunocytochemistry, immunoprecipitation and immunohistochemical procedures. By Western blot, this antibody detects a 10 kDa protein representing S100-alpha from human muscle. Immunohistochemical staining on paraffin sections demonstrates that S100-alpha is localized in serous cells, myoepithelial cells and epithelial cells of intercalated ducts in the parotid gland after staining with PA1-932.
S100 protein is relatively small and, therefore, it is recommended that the electrophoresis be performed using tricine-SDS-PAGE gels and transferred to a nylon membrane.
The PA1-932 immunizing protein corresponds to purified S100-alpha protein from human pectoral muscle cells.
The S100 protein is a low-molecular-weight, acidic and calcium binding protein that in functional form exist in dimers. S100 has two subunits: S100-alpha (94 aa; human chromosome 1) and S100-beta (92 aa; human chromosome 21) that forms as either homodimers (alpha-alpha known as S-100a(0) or beta-beta known as S-100b) or as heterodimers (known as S-100a) of ~21 kDa. S100-alpha and -beta chains show ~58% sequence identity and are both highly conserved among species. S100-alpha was originally believed to be localized to the CNS, but studies have shown it to be found in numerous tissues including cardiac, skeletal and vascular smooth muscle cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
S100A1 and S100B expression patterns identify differentiation status of human articular chondrocytes.
PA1-932 was used in ELISA, immunocytochemistry, and western blot to study the differentiation status of human articular chondrocytes using S100A1 and S100B as biomarkers
|Diaz-Romero J,Quintin A,Schoenholzer E,Pauli C,Despont A,Zumstein MA,Kohl S,Nesic D||Journal of cellular physiology (229:1106)||2014|
Comparative proteomics profiling of a phospholamban mutant mouse model of dilated cardiomyopathy reveals progressive intracellular stress responses.
PA1-932 was used in western blot to perform the global proteomics surveys of cardiac ventricle of a phospholamban mutant mouse
|Gramolini AO,Kislinger T,Alikhani-Koopaei R,Fong V,Thompson NJ,Isserlin R,Sharma P,Oudit GY,Trivieri MG,Fagan A,Kannan A,Higgins DG,Huedig H,Hess G,Arab S,Seidman JG,Seidman CE,Frey B,Perry M,Backx PH,Liu PP,MacLennan DH,Emili A||Molecular and cellular proteomics : MCP (7:519)||2008|