Invitrogen

S100A12 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

View all (12) S100A12 antibodies

Datasheet

Datasheet

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S100A12 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of S100A12 in human bone marrow and cerebral cortex tissues using S100A12 Polyclonal Antibody (Product # PA5-51762). Corresponding S100A12 RNA-seq data are presented for the same tissues.

S100A12 Antibody in Western Blot (WB) — Western blot analysis of S100A12 in control (vector only transfected HEK293T lysate) and S100A12 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a S100A12 Polyclonal Antibody (Product # PA5-51762).

S100A12 Antibody in Relative expression — Relative expression in different tissues in IHC: Detection of differential expression levels of S100A12 demonstrates antibody specificity. Immunohistochemical analysis of S100A12 using anti-S100A12 Polyclonal Antibody (Product # PA5-51762), shows significant staining of S100A12 in human bone marrow and shows minimal or weak staining in human cerebral cortex tissues. The relative expression levels of S100A12 within each tissue is shown using RNA-Seq. {RE}

Product Details

Tested Applications

Dilution

Immunohistochemistry (IHC)

1:500-1:1000

Western Blot (WB)

0.4 µg/mL

Product Specifications

Species Reactivity

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human S100A12

Conjugate

Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2646892

Product Specific Information

Immunogen sequence: KLEEHLEGIV NIFHQYSVRK GHFDTLSKGE LKQLLTKELA NTIKNIKDKA VIDEIFQGLD ANQDEQVDFQ EFISLVAIAL KAAHYHTHKE

Highest antigen sequence identity to the following orthologs: Mouse - 40%, Rat - 40%.

Target Information

S100A12 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A12 is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities. S100A12 is also a ligand for RAGE. Interaction with RAGE on mononuclear phagocytes, lymphocytes and endothelium triggers cellular activation. Nuclear factor kappa B, a key transcription factor for inflammatory events, is activated by S100A12/RAGE. S100A12 has also been shown to have anti-microbial properties.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CAAF1; CAGC; Calcitermin; calcium binding protein in amniotic fluid; Calcium-binding protein in amniotic fluid 1; calgranulin C; Calgranulin-C; CGRP; EN-RAGE; Extracellular newly identified RAGE-binding protein; Migration inhibitory factor-related protein 6; MRP-6; Neutrophil S100 protein; Protein S100-A12; S100 calcium-binding protein A12; S100 calcium-binding protein A12 (calgranulin C)

Gene Aliases: CAAF1; CAGC; CGRP; ENRAGE; MRP-6; MRP6; p6; S100A12

UniProt ID: (Human) P80511

Entrez Gene ID: (Human) 6283

Molecular Function: calcium-binding protein calmodulin intracellular calcium-sensing protein signaling molecule