Invitrogen

S100A8 Polyclonal Antibody

View all (27) S100A8 antibodies

Datasheet

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Datasheet

Protocols

Questions & Answers

Tech Support

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S100A8 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemical analysis of S100A8 in human skin cancer using a S100A8 Polyclonal antibody (Product # PA5-120346). Blue: DAPI for nuclear staining.

S100A8 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of S100A8 in human liver using a Polyclonal antibody (Product # PA5-120346) at a dilution of 1:200.

S100A8 Antibody in Western Blot (WB) — Western Blot analysis of S100A8 in various cell lines using a S100A8 Polyclonal antibody (Product # PA5-120346) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 90s.

Product Details

PA5-120346

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500 - 1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

1:50 - 1:100

Immunocytochemistry (ICC/IF)

1:50 - 1:100

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-93 of human S100A8 (NP_002955.2).

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

4.25 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2913918

Product Specific Information

Positive test controls include: THP-1, Mouse lung, Mouse kidney. The target is usually found in the following locations: Cell membrane, Cytoplasm, Peripheral membrane protein, Secreted, cytoskeleton.

Target Information

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: calgranulin A; Calgranulin-A; Calprotectin L1L subunit; CFAG; Chemotactic cytokine CP-10; chemotactic S100 protein; Cystic fibrosis antigen; Leukocyte L1 complex light chain; Migration inhibitory factor-related protein 8; MRP-8; p8; Pro-inflammatory S100 cytokine; Protein S100-A8; S100 A8; S100 calcium binding protein A8 (calgranulin A); S100 calcium-binding protein A8; S100 calcium-binding protein A8 (calgranulin A); Urinary stone protein band A

Gene Aliases: 60B8AG; AI323541; B8Ag; CAGA; CFAG; CGLA; CP-10; L1Ag; MA387; MIF; MRP8; NIF; P8; S100A8

UniProt ID: (Human) P05109, (Rat) P50115, (Mouse) P27005

Entrez Gene ID: (Human) 6279, (Rat) 116547, (Mouse) 20201

Molecular Function: calmodulin-related