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S1PR3 (EDG3) (extracellular) Antibody (ASR-013-200UL) in WB
Western blot analysis of rat heart (lanes 1 and 3), rat kidney (lanes 2 and 4),human Jurkat acute T cell leukemia (lanes 5 and 7) and mouseWEHI B cell lymphoma (lanes 6 and 8) lysates: - 1,2,5,6. Anti-S1PR3 (EDG3) (extracellular) Antibody (#ASR-013), (1:200).3,4,7,8. Anti-S1PR3 (EDG3) (extracellular) Antibody , preincubated with S1PR3/EDG3 (extracellular) Blocking Peptide (#BLP-SR013).
Product Details
ASR-013-200UL
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
(C)DYVGKLAGRLRD, corresponding to amino acid residues 23-34 of mouse S1PR3, Extracellular, N-terminus
Conjugate
Form
Lyophilized
Concentration
0.8 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
EDG-3 belongs to a family of G-protein coupled receptors whose ligands are lysophospholipids. The ligand for EDG-3 is sphingosine-1-phosphate. There are 8 known members of the EDG receptor family and they are implicated in mediating growth related effects such as induction of cellular proliferation, alterations in differentiation and survival and suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. They couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C and several protein tyrosine kinases. EDG-3 is expressed in cardiovascular, leukocyte-containing and other tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cardiac sphingosine-1-phosphate specific receptor; Endothelial differentiation G-protein coupled receptor 3; endothelial differentiation sphingolipid G-protein-coupled receptor 3; endothelial differentiation, sphingolipid G-protein-coupled receptor, 3; FLJ37523; FLJ93220; G protein-coupled receptor, endothelial differentiation gene-3; Lysophospholipid receptor B3; MGC71696; S1P receptor 3; S1P receptor Edg-3; S1P receptor EDG3; S1P receptor S1P3; sphingosine; Sphingosine 1-phosphate receptor 3; Sphingosine 1-phosphate receptor Edg-3
Gene Aliases: AI132464; C9orf108; C9orf47; EDG-3; EDG3; Edg3l; LPB3; S1P3; S1PR3
UniProt ID: (Human) Q99500, (Mouse) Q9Z0U9
Entrez Gene ID: (Human) 1903, (Mouse) 13610, (Rat) 306792
cytokine production
sphingosine-1-phosphate signaling pathway
inflammatory response
G-protein coupled receptor signaling pathway
adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
Notch signaling pathway
positive regulation of cell proliferation
anatomical structure morphogenesis
regulation of interleukin-1 beta production
negative regulation of establishment of endothelial barrier
signal transduction
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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