|Tested species reactivity||Bovine, Dog, Human, Mouse, Rat|
|Host / Isotype||Goat / IgG|
|Immunogen||synthetic peptide MISKRPEHLRMNL, corresponding to amino acids 490-502 of S6K1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay-dependent|
|Western Blot (WB)||0.2-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-31167 detects S6K1 from human, mouse, rat, dog and cow samples.
PA1-31167 has been successfully used in Western blot applications.
The PA1-31167 immunogen is a synthetic peptide, MISKRPEHLRMNL, corresponding to amino acids 490-502 of S6K1.
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.