|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||An 18 amino acid peptide near the carboxy terminus of human SAE2.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||4 µg/ml|
|Immunofluorescence (IF)||5 ug/ml|
|Western Blot (WB)||0.25 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control is 293 cell lysate.
PA5-20958 can be used with blocking peptide PEP-1072.
Small ubiquitin-like modifiers (SUMOs) are a family of small, related proteins (SUMO-1/2/3/4) that can be enzymatically attached to a target protein by a post-translational modification process termed sumoylation, a major regulator of protein function in cellular processes such as nuclear transport, transcriptional regulation, apoptosis and protein stability. This sumoylation is effected by the heterodimeric enzyme SAE1/SAE2 and the SUMO-1-conjugating enzyme Ubch9. The sumoylation pathway mediated by SAE1/SAE2 is distinct from other ubiquitin-like protein (Ubl) pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.