Western blot analysis of HeLa Whole Cell Lysate using PA5-19679, SAP155 primary antibody at a dilution of 1 ug/ml (lane 1). PA5-19679 staining of Jurkat Whole Cell Lysate at a dilution of 1 ug/ml (lane 2). Blot treated with a secondary IR Dye680-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:10000.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human SAP155.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||1 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1 µg/ml|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
This antibody is predicted to react with mouse and Xenopus laevis based on sequence homology.
This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex . The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Cross-talk between PRMT1-mediated methylation and ubiquitylation on RBM15 controls RNA splicing.
PA5-19679 was used in western blot to report that PRMT1 regulates alternative RNA splicing by reducing RBM15
|Zhang L,Tran NT,Su H,Wang R,Lu Y,Tang H,Aoyagi S,Guo A,Khodadadi-Jamayran A,Zhou D,Qian K,Hricik T,Côté J,Han X,Zhou W,Laha S,Abdel-Wahab O,Levine RL,Raffel G,Liu Y,Chen D,Li H,Townes T,Wang H,Deng H,Zheng YG,Leslie C,Luo M,Zhao X||eLife (4:null)||2015|