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Invitrogen
| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
Assay-dependent | - |
ELISA (ELISA) |
Assay-dependent | - |
Functional Assay (FN) |
Assay-dependent | - |
Control (Ctrl) |
Assay-dependent | - |
| Product Specifications | |
|---|---|
Species |
Virus |
Expression System |
E. coli |
Amino acid sequence |
SARS-CoV-2 Nucleocapsid, Met1-Ala419 with an N-terminal His-tag |
Tag |
His-tag |
Molecular weight |
49.4 kDa |
Class |
Recombinant |
Type |
Protein |
Purity |
>95% by SDS-PAGE; >95% by HPLC |
Endotoxin concentration |
<1.0 EU/µg |
Activity |
Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 Nucleocapsid at 2 μg/mL (100 μL/well) can bind Anti-Nucleocapsid antibody with a linear range of 0.12-1.64 ng/mL. Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 envelope Protein at 2μg/mL (100 μL/well) can bind Recombinant SARS-CoV-2 Nucleocapsid protein, the EC50 of Recombinant SARS-CoV-2 Nucleocapsid protein is 41.10 ng/mL. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1.3 mg/mL |
Purification |
purified |
Storage buffer |
20mM tris, pH 8.0, with 0.1mM EDTA, 500mM NaCl, 10% glycerol |
Contains |
no preservative |
Storage conditions |
-80°C |
Animal Origin Free
Storage: Store the lyophilized protein at -20°C to -80°C for long term. This product is stable at =-70°C for up to 1 year from the date of receipt.
SARS-CoV-2 Nucleocapsid Protein is associated with nucleic acid. It is the most abundant protein for coronavirus. Because of the strong immunogenicity of coronavirus Nucleocapsid, it is believed that SARS-CoV-2 Nucleocapsid Protein has potential value for the diagnosis of the virus. The SARS-CoV-2 N protein is an abundant RNA-binding protein critical for viral genome packaging, yet the molecular details that involve this process are poorly understood. N protein contains three dynamic disordered regions that house putative transiently-helical binding motifs. The two folded domains interact minimally such that full-length N protein is a flexible and multivalent RNA-binding protein. SARS-CoV-2 causes illnesses ranging from the common cold to severe diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: COVID-19 nucleocapsid; COVID19 Nucleocapsid; COVID19 Spike nucleocapsid
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