Purity: > 95% by SDS-PAGE; > 95% by HPLC.
Endotoxin Concentration: < 1.0 EU/µg of the protein by LAL method.
Protein Length/ AA Sequence: Met1-Ala419 of SARS-COV-2(2019-nCoV) Nucleocapsid fused with an 6×His tag at the N-terminus
Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-COV-2 Nucleocapsid at 2 µg/mL (100 µL/well) can bind Anti-Nucleocapsid antibody with a linear range of 0.12-1.64 ng/mL. EC50 = 41.10 ng/mL
Molecular Weight: 49.4 kDa
Animal Origin Free: Yes
Storage: Store the lyophilized protein at -20°C to -80°C for long term. This product is stable at ≤ -70°C for up to 1 year from the date of receipt.
Severe Acute Respiratory Syndrome (SARS), an emerging disease characterized by atypical pneumonia, has recently been attributed to a novel coronavirus (SARS-CoV). The nucleocapsid protein (N) was one of the structural proteins of SARS-CoV (N, S, sE, M). The severe acute respiratory syndrome coronavirus (SARS-CoV) nucleocapsid (N) protein is the most abundant protein in the virus-infected cells. The SARS-CoV-2 S gene has 76% amino acid similarity to the SARS-CoV S gene, and nonsynonymous mutations developed in the S protein as the SARS-CoV epidemic developed, reports have shown that the crystal structure of the SARS-CoV-2 nucleocapsid protein is similar to those of previously described coronavirus N proteins, but their surface electrostatic potential characteristics are distinct.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Coronavirus; COVID; COVID 19; COVID-19; COVID19; SARS; SARS Coronavirus; SARS CoV; SARS CoV-2; SARS CoV2; SARS Nucleocapsid; SARS-CoV-2; SARS-CoV2