Storage: This product is stable at &plusmin; -70°C for up to 6 months from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.
Purity: >97% by SDS-PAGE
Endotoxin: < 0.1 EU/µg of the protein by LAL method.
Source: Recombinant 2019-nCoV S1+S2 ECD (S-ECD) Protein is produced by HEK293 cells expression system.The target protein is expressed with sequence (Val11-Gln1208) of 2019-ncov S1+S2 ECD (S-ECD)(Accession #YP_009724390.1) fused with a 6xHis tag at the C-terminus.
Bioactivity: Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-COV-2 Spike S1+S2 ECD-His at 2 µg/mL (100 µL/well) can bind Recombinant Human ACE2 with a linear range of 0.15-9.92 ng/mL.
SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold symptoms to severe effects including death and respiratory distress. The CoV Spike (S) protein plays the most important roles in viral attachment, fusion and entry, and serves as a target for development of antibodies, entry inhibitors and vaccines. Of note is the Spike protein receptor-binding domain (RBD, S-RBD) in SARS-CoV-2 Spike protein which binds strongly to human and bat angiotensin-converting enzyme 2 (ACE2) receptors. SARS-CoV-2 RBD exhibits significantly higher binding affinity to ACE2 receptor than SARS-CoV RBD in comparison. S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells. SARS-CoV-2 S is 1273 amino acids long and consists of a signal peptide (amino acids 1 13) located at the N-terminus, the S1 subunit (14 685 residues), and the S2 subunit (686 1273 residues); the last two regions are responsible for receptor binding and membrane fusion, respectively.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Coronavirus; COVID; Covid 19; COVID-19; COVID19; SARS; SARS Coronavirus; SARS CoV; SARS CoV-2; SARS CoV2; SARS Spike Protein; SARS-CoV-2; SARS-CoV2