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Invitrogen
SARS-CoV-2 Spike Protein S1 (aa14-683), His-Avi Tag Recombinant Protein
Product Details
RP-87681
| Applications | Tested Dilution | Publications |
|---|---|---|
ELISA (ELISA) |
Assay-dependent | - |
Control (Ctrl) |
Assay-dependent | - |
| Product Specifications | |
|---|---|
Species |
Virus |
Expression System |
HEK293 cells |
Amino acid sequence |
SARS-CoV-2 Spike Protein S1, amino acids Gln14-Arg683 (Accession # YP_009724390.1) with a C-terminal His-tag and Avi-tag |
Tag |
His-tag, Avi-tag |
Molecular weight |
77.7 kDa |
Class |
Recombinant |
Type |
Protein |
Purity |
>95% by SDS-PAGE and HPLC |
Endotoxin concentration |
<1.0 EU/µg |
Activity |
Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 Spike S1 at 2μg/mL (100 μL/well) can bind recombinant Human ACE2 with a linear range of 0.15-6.85 ng/mL. Immobilized Human ACE2 on COOH Chip, can bind SARS-COV-2 Spike S1 with an affinity constant of 24.5 nM as determined in a SPR assay (Nicoya OpenSPR). |
Conjugate |
Unconjugated |
Form |
Lyophilized |
Purification |
SDS-PAGE |
Storage buffer |
PBS, pH 7.4 |
Contains |
no preservative |
Storage conditions |
-20°C or -80°C if preferred |
Product Specific Information
Reconstitution: Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Target Information
SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold symptoms to severe effects including death and respiratory distress. SARS-CoV-2 Spike Protein is composed of S1 domain and S2 domain (Spike Protein). The Spike protein is the main surface antigen of the coronavirus. The glycosylated Spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients. S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells. CoV diversity is reflected in the variable Spike proteins (S proteins), which have evolved into forms differing in their receptor interactions and their response to various environmental triggers of virus-cell membrane fusion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Coronavirus; COVID; Covid 19; COVID-19; COVID19; SARS; SARS Coronavirus; SARS CoV; SARS CoV-2; SARS CoV2; SARS Spike Protein; SARS-CoV-2; SARS-CoV2
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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