Invitrogen

SARS Coronavirus Spike Protein Polyclonal Antibody

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SARS Coronavirus Spike Protein Antibody in Western Blot (WB) — Western blot analysis of SARS Coronavirus Spike Protein in SARS-CoV-1 Spike , SARS CoV-2 Spike , SArS-CoV-2 Spike S2 Subunit. Samples were incubated in SARS Coronavirus Spike Protein polyclonal antibody (Product # PA1-41165) using a dilution of 0.5 µg/mL followed by a HRP-conjugated Anti-Rabbit IgG secondary antibody. A specific band was detected for SARS-CoV-2 Spike S2 Subunit at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions.

Product Details

PA1-41165

Applications	Tested Dilution	Publications
Western Blot (WB)	0.05-1.0 µg/mL	View 6 publications 6 publications
Immunohistochemistry (IHC)	Assay-Dependent	-
Immunocytochemistry (ICC/IF)	Assay-Dependent	-
Flow Cytometry (Flow)	-	View 2 publications 2 publications

Product Specifications
Species Reactivity	Virus
Published species	Virus
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide corresponding to residues 1124-1140 of the putative SARS Spike glycoprotein.
Conjugate	Unconjugated
Form	Liquid
Concentration	1.0 mg/mL
Purification	Protein G
Storage buffer	PBS
Contains	0.05% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_1087210

Product Specific Information

This antibody has been used for Flow Cytometry to detect HEK293T.17 cells transfected with vaccine-expressing membrane-bound SARS-CoV-2 spike protein. View Article

The immunogen is 100% (16/16) homologous to SARS-COV-2 (COVID-19) Spike protein. The cross reactivity is not empirically tested.

Suggested positive control: transfected mouse melanoma cell lysate.

Target Information

It has recently been shown that SARS (severe acute respiratory syndrome) is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness, such as the common cold, in humans. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The glycosylated spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

SARS-CoV-2 spike N-terminal domain modulates TMPRSS2-dependent viral entry and fusogenicity.

Cell reports

Meng B,Datir R,Choi J,Bradley JR,Smith KGC,Lee JH,Gupta RK

PA1-41165 was used in Flow cytometry/Cell sorting to conclude that the NTD allosterically modulates S1/S2 cleavage and spike-mediated functions in a spike context-dependent manner, and allosteric interactions may be lost when combining regions from more distantly related VOCs.

Tue Aug 16 00:00:00 EDT 2022

Altered TMPRSS2 usage by SARS-CoV-2 Omicron impacts infectivity and fusogenicity.

Nature

Meng B,Abdullahi A,Ferreira IATM,Goonawardane N,Saito A,Kimura I,Yamasoba D,Gerber PP,Fatihi S,Rathore S,Zepeda SK,Papa G,Kemp SA,Ikeda T,Toyoda M,Tan TS,Kuramochi J,Mitsunaga S,Ueno T,Shirakawa K,Takaori-Kondo A,Brevini T,Mallery DL,Charles OJ,Bowen JE,Joshi A,Walls AC,Jackson L,Martin D,Smith KGC,Bradley J,Briggs JAG,Choi J,Madissoon E,Meyer KB,Mlcochova P,Ceron-Gutierrez L,Doffinger R,Teichmann SA,Fisher AJ,Pizzuto MS,de Marco A,Corti D,Hosmillo M,Lee JH,James LC,Thukral L,Veesler D,Sigal A,Sampaziotis F,Goodfellow IG,Matheson NJ,Sato K,Gupta RK

PA1-41165 was used in Western Blot to show that the spike protein of Omicron has a higher affinity for ACE2 compared with Delta, and a marked change in its antigenicity increases Omicron's evasion of therapeutic monoclonal and vaccine-elicited polyclonal neutralizing antibodies after two doses.

Tue Mar 01 00:00:00 EST 2022

SARS-CoV-2 B.1.617.2 Delta variant replication and immune evasion.

Nature

Mlcochova P,Kemp SA,Dhar MS,Papa G,Meng B,Ferreira IATM,Datir R,Collier DA,Albecka A,Singh S,Pandey R,Brown J,Zhou J,Goonawardane N,Mishra S,Whittaker C,Mellan T,Marwal R,Datta M,Sengupta S,Ponnusamy K,Radhakrishnan VS,Abdullahi A,Charles O,Chattopadhyay P,Devi P,Caputo D,Peacock T,Wattal C,Goel N,Satwik A,Vaishya R,Agarwal M,Mavousian A,Lee JH,Bassi J,Silacci-Fegni C,Saliba C,Pinto D,Irie T,Yoshida I,Hamilton WL,Sato K,Bhatt S,Flaxman S,James LC,Corti D,Piccoli L,Barclay WS,Rakshit P,Agrawal A,Gupta RK

PA1-41165 was used in Western Blot to observe that B.1.617.2 had higher replication and spike-mediated entry than B.1.617.1, potentially explaining the B.1.617.2 dominance.

Mon Nov 01 00:00:00 EDT 2021

TMPRSS2 expression dictates the entry route used by SARS-CoV-2 to infect host cells.

The EMBO journal

Koch J,Uckeley ZM,Doldan P,Stanifer M,Boulant S,Lozach PY

PA1-41165 was used in Western Blot to indicate that SARS-CoV-2 infects cells through distinct, mutually exclusive entry routes and highlight the importance of TMPRSS2 for SARS-CoV-2 sorting into either pathway.

Mon Aug 16 00:00:00 EDT 2021

Recurrent emergence of SARS-CoV-2 spike deletion H69/V70 and its role in the Alpha variant B.1.1.7.

Cell reports

Meng B,Kemp SA,Papa G,Datir R,Ferreira IATM,Marelli S,Harvey WT,Lytras S,Mohamed A,Gallo G,Thakur N,Collier DA,Mlcochova P,Duncan LM,Carabelli AM,Kenyon JC,Lever AM,De Marco A,Saliba C,Culap K,Cameroni E,Matheson NJ,Piccoli L,Corti D,James LC,Robertson DL,Bailey D,Gupta RK

PA1-41165 was used in Western Blot to report severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike ΔH69/V70 in multiple independent lineages, often occurring after acquisition of receptor binding motif replacements such as N439K and Y453F, known to increase binding affinity to the ACE2 receptor and confer antibody escape.

Tue Jun 29 00:00:00 EDT 2021

Furin cleavage of SARS-CoV-2 Spike promotes but is not essential for infection and cell-cell fusion.

PLoS pathogens

Papa G,Mallery DL,Albecka A,Welch LG,Cattin-Ortolá J,Luptak J,Paul D,McMahon HT,Goodfellow IG,Carter A,Munro S,James LC

PA1-41165 was used in Western Blotting to show that CRISPR-Cas9 knockout of furin reduces, but does not prevent, the production of infectious SARS-CoV-2 virus.

Fri Jan 01 00:00:00 EST 2021

SARS-CoV-2 growth, furin-cleavage-site adaptation and neutralization using serum from acutely infected hospitalized COVID-19 patients.

The Journal of general virology

Klimstra WB,Tilston-Lunel NL,Nambulli S,Boslett J,McMillen CM,Gilliland T,Dunn MD,Sun C,Wheeler SE,Wells A,Hartman AL,McElroy AK,Reed DS,Rennick LJ,Duprex WP

Published figure using SARS Coronavirus Spike Protein polyclonal antibody (Product # PA1-41165) in Western Blot

Sun Nov 01 00:00:00 EDT 2020

SARS-CoV-2 growth, furin-cleavage-site adaptation and neutralization using serum from acutely infected hospitalized COVID-19 patients.

The Journal of general virology

Klimstra WB,Tilston-Lunel NL,Nambulli S,Boslett J,McMillen CM,Gilliland T,Dunn MD,Sun C,Wheeler SE,Wells A,Hartman AL,McElroy AK,Reed DS,Rennick LJ,Duprex WP

Published figure using SARS Coronavirus Spike Protein polyclonal antibody (Product # PA1-41165) in Western Blot

Sun Nov 01 00:00:00 EDT 2020

Self-amplifying RNA SARS-CoV-2 lipid nanoparticle vaccine candidate induces high neutralizing antibody titers in mice.

Nature communications

McKay PF,Hu K,Blakney AK,Samnuan K,Brown JC,Penn R,Zhou J,Bouton CR,Rogers P,Polra K,Lin PJC,Barbosa C,Tam YK,Barclay WS,Shattock RJ

PA1-41165 was used in Flow cytometry/Cell sorting to present a self-amplifying RNA encoding the SARS-CoV-2 spike protein encapsulated within a lipid nanoparticle (LNP) as a vaccine.

Thu Jul 09 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: Coronavirus; COVID; Covid 19; COVID-19; COVID19; SARS; SARS Coronavirus; SARS CoV; SARS CoV-2; SARS CoV2; SARS Spike Protein; SARS-CoV-2; SARS-CoV2

SARS Coronavirus Spike Protein Polyclonal Antibody

Product Details

PA1-41165

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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