|Tested species reactivity||Amphibian, Rat, Fruit fly, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Partially purified mammalian spliceosomes.|
|Contains||15mM sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay-dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The antibody labels SC-35 as a speckled pattern in the nucleoplasm, excluding the nucleoli.
Other uses include inhibition and depletion of splicing activity in nuclear extracts and immunoaffinity purification.
No antigen retrieval is necessary.Block in normal serum 10% in TBS (pH 7.6) for 30 min followed by incubation overnight at 4C.
Use ABC amplification- DAB detection.
In Western blot, this antibody detects a doublet of approximately 35 kDa.
Suggested positive control: cultured human fibroblasts.
SC35 is involved in pre-mRNA splicing and is found in the bodies in the nucleus referred to as speckles, SC 35 domains or splicing factor compartments (SFCs). The SC-35 clone is frequently used as a marker for these bodies, the role of which is controversial. Speckles correspond largely if not entirely to ultrastructures termed interchromatin granule clusters (IGCs). SC35 is excluded from the nucleolus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.