|Tested species reactivity||Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: C N(217) Q P Q T Q Y S A T P N Y T Y S N(233)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
PA1-737 detects secretory carrier membrane protein 5 (SCAMP 5) from rat brain extract.
PA1-737 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~25 kDa protein representing SCAMP 5 from rat brain extract.
PA1-737 immunizing peptide corresponds to amino acid residues 217-233 from rat SCAMP 5. This sequence is 94% conserved in mouse. PA1-737 immunizing peptide (Cat. # PEP-152) is available for use in neutralization and control experiments.
Secretory carrier membrane proteins (SCAMPs) are proteins that are components of post-Golgi membranes. These proteins are implicated to function in membrane trafficking. In fibroblasts, SCAMPs are concentrated in compartments that are involved in the recycling of cell surface receptors and endocytosis. In neurons, SCAMPs are associated with synaptic vesicles, secretion granules and transporter vesicles. SCAMPs are composed of four central transmembrane regions and a cytoplasmic tail. Of the five known SCAMPs, SCAMPs 1-3 contain cytoplasmic N-terminal regions with NPF repeats. NPF repeats are found to interact with EH domain proteins that function in budding of transport vesicles from the plasma membrane or the Golgi complex. SCAMPs 4 and 5 lack the N-terminal NPF repeats. SCAMPs 1-4 are all ubiquitously coexpressed while SCAMP 5 is only detectable in the brain. Studies show that SCAMP 5 is expressed late in development which is coincident with expansion of mature synapses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Tissue expression of the vesicle protein pantophysin.
PA1-737 was used in immunohistochemistry and western blot to study the expression and distribution of the vesicle protein pantophysin
|Windoffer R,Borchert-Stuhlträger M,Haass NK,Thomas S,Hergt M,Bulitta CJ,Leube RE||Cell and tissue research (296:499)||1999|