Invitrogen

SCN3B Monoclonal Antibody (S396-29), APC

Product Image Gallery

SCN3B Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with Nav beta 3 monoclonal antibody (Product # MA5-45737) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 for 60 min RT, 5 min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Nav beta 3 antibody. (D) Composite.

SCN3B Antibody in Western Blot (WB) — Western Blot analysis of Mouse Brain showing detection of ~40 kDa Nav Beta 3 protein. Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 µg. Samples were incubated with Nav Beta 3 monoclonal antibody (Product # MA5-45737) at 1:1,000 for 16 hours at 4°C, followed by Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~40 kDa.

Product Details

MA5-45737

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunocytochemistry (ICC/IF)

1:100

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

S396-29

Immunogen

Fusion protein amino acids 1-215 (full-length) of rat NavBeta3

View immunogen

Conjugate

APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

47.35mM phosphate/2.15mM MES, pH 7.4, with 1.033mM EDTA

Contains

no preservative

Storage conditions

4° C

Target Information

Voltage-gated sodium channels are transmembrane glycoprotein complexes composed of a large alpha subunit and one or more regulatory beta subunits. They are responsible for the generation and propagation of action potentials in neurons and muscle. This gene encodes one member of the sodium channel beta subunit gene family, and influences the inactivation kinetics of the sodium channel. Two alternatively spliced variants, encoding the same protein, have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: brain and heart sodium channel beta 3 subunit; sodium channel beta 3 subunit; Sodium channel subunit beta-3; sodium channel, voltage gated, type III beta subunit; sodium channel, voltage-gated, type III, beta subunit; voltage-gated sodium channel beta-3 subunit

Gene Aliases: 1110001K16Rik; 4833414B02Rik; ATFB16; BRGDA7; HSA243396; KIAA1158; SCN3B; SCNB3

UniProt ID: (Human) Q9NY72, (Mouse) Q8BHK2, (Rat) Q9JK00

Entrez Gene ID: (Human) 55800, (Mouse) 235281, (Rat) 245956

Molecular Function: ion channel transporter voltage-gated ion channel