|Tested species reactivity||Bovine, C. elegans, Human, Mouse, Rat|
|Published species reactivity||Rat, Mouse, Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Bovine Heart Complex II, with traces of Complex III|
|Storage buffer||HEPES buffered saline|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Western Blot (WB)||1.0 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
SDHA is an important component in the citric acid cycle and respiratory chain. SDHA converts succinate to fumarate, transfers electrons in the respiratory chain, and ultimately transfers electrons to ubiquinone. SDHA is localized within the mitochondrial inner membrane. Mutations in the gene have been associated with Leigh syndrome and Huntington's disease. However, SDHA also appears to have tumor suppressor effects.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A ketogenic diet accelerates neurodegeneration in mice with induced mitochondrial DNA toxicity in the forebrain.
459200 was used in immunohistochemistry - paraffin section to test if the severe neurodegeneration observed in mutUNG1-expressing mice is prevented by a ketogenic diet
|Lauritzen KH,Hasan-Olive MM,Regnell CE,Kleppa L,Scheibye-Knudsen M,Gjedde A,Klungland A,Bohr VA,Storm-Mathisen J,Bergersen LH||Neurobiology of aging (48:34)||2016|
Mitochondrial DNA toxicity compromises mitochondrial dynamics and induces hippocampal antioxidant defenses.
459200 was used in immunohistochemistry - paraffin section to investigate the effects of mitochondrial DNA damage on hippocampal neurons.
|Lauritzen KH,Cheng C,Wiksen H,Bergersen LH,Klungland A||DNA repair (10:639)||2011|
Mutant desmin substantially perturbs mitochondrial morphology, function and maintenance in skeletal muscle tissue.
459200 was used in western blot show that mutant desmin expression results in mitochondrial damage during early stages of desminopathies
|Winter L,Wittig I,Peeva V,Eggers B,Heidler J,Chevessier F,Kley RA,Barkovits K,Strecker V,Berwanger C,Herrmann H,Marcus K,Kornblum C,Kunz WS,Schröder R,Clemen CS||Acta neuropathologica (132:453)||2016|
Loss of CLPP alleviates mitochondrial cardiomyopathy without affecting the mammalian UPRmt.
459200 was used in western blot to study the alleviation of mitochondrial cardiomyopathy without affecting the mammalian UPRmt due to loss of CLPP
|Seiferling D,Szczepanowska K,Becker C,Senft K,Hermans S,Maiti P,König T,Kukat A,Trifunovic A||EMBO reports (17:953)||2016|
|Not Applicable||Not Cited||
Downstream effects of plectin mutations in epidermolysis bullosa simplex with muscular dystrophy.
459200 was used in western blot to study muscular dystrophy and the downstream effects of plectin mutations in epidermolysis bullosa simplex
|Winter L,Türk M,Harter PN,Mittelbronn M,Kornblum C,Norwood F,Jungbluth H,Thiel CT,Schlötzer-Schrehardt U,Schröder R||Acta neuropathologica communications (4:null)||2016|
The mitochondrial function of the cerebral vasculature in insulin-resistant Zucker obese rats.
459200 was used in western blot to study cerebral vasculature mitochondrial function in insulin-resistant Zucker obese rats
|Merdzo I,Rutkai I,Tokes T,Sure VN,Katakam PV,Busija DW||American journal of physiology. Heart and circulatory physiology (310:H830)||2016|
Reduction in mitochondrial iron alleviates cardiac damage during injury.
459200 was used in western blot to elucidate how a reduction in mitochondrial iron during injury can alleviate cardiac damage
|Chang HC,Wu R,Shang M,Sato T,Chen C,Shapiro JS,Liu T,Thakur A,Sawicki KT,Prasad SV,Ardehali H||EMBO molecular medicine (8:247)||2016|
Mitochondrial protein hyperacetylation in the failing heart.
459200 was used in western blot to characterize a failing heart and mitochondrial protein hyperacetylation
|Horton JL,Martin OJ,Lai L,Riley NM,Richards AL,Vega RB,Leone TC,Pagliarini DJ,Muoio DM,Bedi KC,Margulies KB,Coon JJ,Kelly DP||JCI insight (2:null)||2016|
|Not Applicable||Not Cited||
Loss of OMA1 delays neurodegeneration by preventing stress-induced OPA1 processing in mitochondria.
459200 was used in western blot to analyze delay of neurodegeneration by preventing stress-induced OPA1 processing in mitochondria by loss of OMA1
|Korwitz A,Merkwirth C,Richter-Dennerlein R,Tröder SE,Sprenger HG,Quirós PM,López-Otín C,Rugarli EI,Langer T||The Journal of cell biology (212:157)||2016|
Imbalanced OPA1 processing and mitochondrial fragmentation cause heart failure in mice.
459200 was used in western blot to study how OMA1 links mitochondrial morphology and cardiac metabolism.
|Wai T,García-Prieto J,Baker MJ,Merkwirth C,Benit P,Rustin P,Rupérez FJ,Barbas C,Ibañez B,Langer T||Science (New York, N.Y.) (350:null)||2015|
Dynamics of enhanced mitochondrial respiration in female compared with male rat cerebral arteries.
459200 was used in western blot to test if mitochondrial energetics of large cerebral arteries are sex dependent.
|Rutkai I,Dutta S,Katakam PV,Busija DW||American journal of physiology. Heart and circulatory physiology (309:H1490)||2015|
AMP-activated protein kinase controls exercise training- and AICAR-induced increases in SIRT3 and MnSOD.
459200 was used in western blot to test the requirement of AMP-activated protein kinase for exercise training-induced increases in skeletal muscle abundance of mitochondrial proteins.
|Brandauer J,Andersen MA,Kellezi H,Risis S,Frøsig C,Vienberg SG,Treebak JT||Frontiers in physiology (6:null)||2015|
CNC-bZIP protein Nrf1-dependent regulation of glucose-stimulated insulin secretion.
459200 was used in western blot to assess effects nuclear factor-erythroid 2-related factor 1 deficiency in beta-cells on beta-cell function and glucose homeostasis
|Zheng H,Fu J,Xue P,Zhao R,Dong J,Liu D,Yamamoto M,Tong Q,Teng W,Qu W,Zhang Q,Andersen ME,Pi J||Antioxidants and redox signaling (22:819)||2015|
Overexpression of TFAM or twinkle increases mtDNA copy number and facilitates cardioprotection associated with limited mitochondrial oxidative stress.
459200 was used in western blot to assess the role of mtDNA copy number in heart failure
|Ikeda M,Ide T,Fujino T,Arai S,Saku K,Kakino T,Tyynismaa H,Yamasaki T,Yamada K,Kang D,Suomalainen A,Sunagawa K||PloS one (10:null)||2015|
Cytochrome c oxidase deficiency accelerates mitochondrial apoptosis by activating ceramide synthase 6.
459200 was used in western blot to elucidate the molecular mechanisms by which mitochondrial respiratory chain dysfunction results in cell death
|Schüll S,Günther SD,Brodesser S,Seeger JM,Tosetti B,Wiegmann K,Pongratz C,Diaz F,Witt A,Andree M,Brinkmann K,Krönke M,Wiesner RJ,Kashkar H||Cell death and disease (6:null)||2015|
Identification of mitochondrial dysfunction in Hutchinson-Gilford progeria syndrome through use of stable isotope labeling with amino acids in cell culture.
459200 was used in western blot to elucidate the mechanisms underlying cellular damage and senescence and accelerated aging in Hutchinson-Gilford progeria syndrome.
|Rivera-Torres J,Acín-Perez R,Cabezas-Sánchez P,Osorio FG,Gonzalez-Gómez C,Megias D,Cámara C,López-Otín C,Enríquez JA,Luque-García JL,Andrés V||Journal of proteomics (91:466)||2013|
Mitochondrial dynamics associated with oxygen-glucose deprivation in rat primary neuronal cultures.
459200 was used in western blot to investigate the mitochondrial dynamics in rat neurons following oxygen-glucose deprivation
|Wappler EA,Institoris A,Dutta S,Katakam PV,Busija DW||PloS one (8:null)||2013|
Irgm1 (LRG-47), a regulator of cell-autonomous immunity, does not localize to mycobacterial or listerial phagosomes in IFN-¿-induced mouse cells.
459200 was used in immunocytochemistry to determine if Irgm targets mycobacterial and listerial phagosomes.
|Springer HM,Schramm M,Taylor GA,Howard JC||Journal of immunology (Baltimore, Md. : 1950) (191:1765)||2013|
Identification of human fumarylacetoacetate hydrolase domain-containing protein 1 (FAHD1) as a novel mitochondrial acylpyruvase.
459200 was used in immunocytochemistry to determine the enzymatic function of FAHD1.
|Pircher H,Straganz GD,Ehehalt D,Morrow G,Tanguay RM,Jansen-Dürr P||The Journal of biological chemistry (286:36500)||2011|