ICC application requires heat-induced antigen retrieval where aldehydes are used as fixatives. Use 20min incubation at 90-100°C in 0.1 M Tris/HCl pH 9.5 with 5% urea (wt/vol).
Positive control: Human heart mitochondria.
This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29.
Protein Aliases: Flavoprotein subunit of complex II; Fp; succinate dehydrogenase [ubiquinone] flavoprotein subunit; Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial; succinate dehydrogenase complex subunit A; succinate dehydrogenase complex subunit A, flavoprotein (Fp); succinate dehydrogenase complex, subunit A, flavoprotein (Fp); succinate dehydrogenase flavoprotein subunit A
Gene Aliases: 1500032O14Rik; 2310034D06Rik; 4921513A11; BOS_19942; C81073; CMD1GG; FP; PGL5; SDH1; SDH2; SDHA; SDHF; SDHFP1