ICC application requires heat-induced antigen retrieval where aldehydes are used as fixatives. Use 20min incubation at 90-100°C in 0.1 M Tris/HCl pH 9.5 with 5% urea (wt/vol).|Positive control: Human heart mitochondria.
SDHA is an important component in the citric acid cycle and respiratory chain. SDHA converts succinate to fumarate, transfers electrons in the respiratory chain, and ultimately transfers electrons to ubiquinone. SDHA is localized within the mitochondrial inner membrane. Mutations in the gene have been associated with Leigh syndrome and Huntington's disease. However, SDHA also appears to have tumor suppressor effects.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CMD1GG; Flavoprotein subunit of complex II; Fp; mitochondrial; PGL5; SDH1; SDH2; SDHF; succinate dehydrogenase [ubiquinone] flavoprotein subunit; Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial; succinate dehydrogenase complex flavoprotein subunit; succinate dehydrogenase complex subunit A; succinate dehydrogenase complex subunit A, flavoprotein (Fp); succinate dehydrogenase complex, subunit A, flavoprotein (Fp); succinate dehydrogenase flavoprotein subunit A
Gene Aliases: 1500032O14Rik; 2310034D06Rik; 4921513A11; BOS_19942; C81073; CMD1GG; FP; PGL5; SDH1; SDH2; SDHA; SDHF; SDHFP1