|Tested species reactivity||Bovine, Human, Mouse, Rat, Zebrafish|
|Published species reactivity||Rat, Mouse, Human, Not Applicable|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||Bovine Heart Complex II, with traces of Complex III.|
|Storage buffer||HEPES buffered saline|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||5 ug/ml|
|Immunofluorescence (IF)||Assay Dependent|
|Western Blot (WB)||5 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The transcriptional coregulator PGC-1ß controls mitochondrial function and anti-oxidant defence in skeletal muscles.
459230 was used in western blot to find the function of transcriptional coregulator PGC-1beta in mitochondria and anti-oxidant defense in skeletal muscles
|Gali Ramamoorthy T,Laverny G,Schlagowski AI,Zoll J,Messaddeq N,Bornert JM,Panza S,Ferry A,Geny B,Metzger D||Nature communications (6:null)||2015|
Prevention and reversal of severe mitochondrial cardiomyopathy by gene therapy in a mouse model of Friedreich's ataxia.
459230 was used in western blot to assess the use of gene therapy in treating Friedreich's ataxia cardiomyopathy in a mouse model.
|Perdomini M,Belbellaa B,Monassier L,Reutenauer L,Messaddeq N,Cartier N,Crystal RG,Aubourg P,Puccio H||Nature medicine (20:542)||2014|
ß-Adrenergic stimulation does not activate p38 MAP kinase or induce PGC-1¿ in skeletal muscle.
459230 was used in western blot to test if the increases in PGC-α and mitochondrial biogenesis induced by endurance exercise are mediated by catecholamines.
|Kim SH,Asaka M,Higashida K,Takahashi Y,Holloszy JO,Han DH||American journal of physiology. Endocrinology and metabolism (304:E844)||2013|
High-throughput screening for growth inhibitors using a yeast model of familial paraganglioma.
459230 was used in western blot to identify compounds that are toxic to succinate dehydrogenase mutant cells using a yeast model of paraganglioma.
|Bancos I,Bida JP,Tian D,Bundrick M,John K,Holte MN,Her YF,Evans D,Saenz DT,Poeschla EM,Hook D,Georg G,Maher LJ||PloS one (8:null)||2013|
Independent roles of methionine sulfoxide reductase A in mitochondrial ATP synthesis and as antioxidant in retinal pigment epithelial cells.
459230 was used in western blot to test if and how MsrA affects retinal pigment epithelium functionality.
|Dun Y,Vargas J,Brot N,Finnemann SC||Free radical biology and medicine (65:1340)||2013|