|Tested species reactivity||Mouse, Rat|
|Published species reactivity||Dog, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: V(29) T D A R E R Y G P N(39).|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-910A detects sarcoplasmic or endoplasmic reticulum calcium ATPase 3 (SERCA3) mouse and rat samples. This antibody does not detect other SERCA isoforms.
PA1-910A has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~97 kDa protein representing recombinant SERCA3 from rat samples.
PA1-910A immunizing peptide corresponds to amino acid residues 29-39 from rat and mouse SERCA3. PA1-910A immunizing peptide (Cat. # PEP-015) is available for neutralization and control experiments.
ATP dependent calcium pumps are responsible in part for the maintenance of low cytoplasmic free calcium concentrations. The ATP pumps that reside in intracellular organelles are encoded by a family of structurally related enzymes, termed the sarcoplasmic or endoplasmic reticulum calcium ATPases (SERCA). The SERCA2 gene is subject to tissue dependent processing which is responsible for the generation of SERCA2a muscle-specific isoform expressed in type I (slow) skeletal, cardiac and smooth muscle and the SERCA2b isoform expressed in all cell types. SERCA3 has been found to co-express with SERCA2b in platelets, mast cells, lymphoid cells, and epithelial cells. Due to this pattern of expression it is referred to as the non-muscle form of SERCA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Slowed relaxation and preserved maximal force in soleus muscles of mice with targeted disruption of the Serca2 gene in skeletal muscle.
PA1-910A was used in western blot to investigate the role of SERCAs in muscle contractile function
|Sjåland C,Lunde PK,Swift F,Munkvik M,Ericsson M,Lunde M,Boye S,Christensen G,Ellingsen Ø,Sejersted OM,Andersson KB||The Journal of physiology (589:6139)||2011|
Immunohistochemical evidence for expression of fast-twitch type sarco(endo)plasmic reticulum Ca2+ ATPase (SERCA1) in German shepherd dogs with dilated cardiomyopathy myocardium.
PA1-910A was used in immunohistochemistry to investigate the level of SERCA1 in dilated cardiomyopathy canine myocardium
|Summerfield N,Peters ME,Hercock CA,Mobasheri A,Young IS||Journal of veterinary cardiology : the official journal of the European Society of Veterinary Cardiology (12:17)||2010|