|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||His tagged fusion protein corresponding to full length human PEDF (Pigment Epithelial Derived Factor) expressed in baby hamster kidney cells.|
|Storage buffer||0.1M tris glycine, pH 7.4, with 0.15M NaCl, 30% glycerol|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.5-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-29882 detects SERPINF1 from human, mouse samples.
PA1-29882 has been successfully used in Western blot applications.
The PA1-29882 immunogen is His tagged fusion protein corresponding to full length human PEDF (Pigment Epithelial Derived Factor) expressed in baby hamster kidney cells.
Pigment epithelium derived factor, originally identified in conditioned medium of cultured human fetal retinal pigment epithelial (RPE) cells, is a neurotrophic protein that induces extensive neuronal differentiation in human Y79 retinoblastoma cells, a neoplastic counterpart of normal retinoblasts. It has been suggested that PEDF is synthesized by RPE cells and secreted into the retina interphotoreceptor matrix where it may influence development/differentiation of the neural retina. PEDF is a potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. The PEDF gene is a member of the serpin gene family. Serpins are a group of serine protease inhibitors, some of which have also been reported to exhibit neurotrophic activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.