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Western blot analysis was performed on whole cell extracts (30 µg lysate) of PC-3 (Lane 1), NTERA-2 (Lane 2), A-431 (Lane 3), MCF7 (Lane 4), Caco-2 (Lane 5), A549 ( Lane 6), SH-SY5Y (Lane 7) and HeLa (Lane 8). The blots were probed with Anti-SF2 / ASF Mouse Monoclonal Antibody (Product # 32-4500, 2 µg/ml) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjµgate (Product # A28177, 0.4 µg/ml, 1:2500 dilution). A 32 kDa band corresponding to SF2 / ASF was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Human, Mouse|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Recombinant SF2/ASF protein.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunofluorescence (IF)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This gene encodes a member of the arginine/serine-rich splicing factor protein family, and functions in both constitutive and alternative pre-mRNA splicing. The protein binds to pre-mRNA transcripts and components of the spliceosome, and can either activate or repress splicing depending on the location of the pre-mRNA binding site. The protein's ability to activate splicing is regulated by phosphorylation and interactions with other splicing factor associated proteins. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
SRSF1 and hnRNP H antagonistically regulate splicing of COLQ exon 16 in a congenital myasthenic syndrome.
32-4500 was used in western blot to characterize acetylcholinesterase mutations.
|Rahman MA,Azuma Y,Nasrin F,Takeda J,Nazim M,Bin Ahsan K,Masuda A,Engel AG,Ohno K||Scientific reports (5:null)||2015|
Splice-shifting oligonucleotide (SSO) mediated blocking of an exonic splicing enhancer (ESE) created by the prevalent c.903+469T>C MTRR mutation corrects splicing and restores enzyme activity in patient cells.
32-4500 was used in western blot to investigate the therapeutic potential of splicinge-shifting oligonucleotide in the cblE type of homocystinuria
|Palhais B,Præstegaard VS,Sabaratnam R,Doktor TK,Lutz S,Burda P,Suormala T,Baumgartner M,Fowler B,Bruun GH,Andersen HS,Kožich V,Andresen BS||Nucleic acids research (43:4627)||2015|
Human Papillomavirus 16 Oncoprotein Expression Is Controlled by the Cellular Splicing Factor SRSF2 (SC35).
32-4500 was used in western blot to study the regulation of the human papillomavirus 16 oncoprotein expression
|McFarlane M,MacDonald AI,Stevenson A,Graham SV||Journal of virology (89:5276)||2015|
Eight nucleotide substitutions inhibit splicing to HPV-16 3'-splice site SA3358 and reduce the efficiency by which HPV-16 increases the life span of primary human keratinocytes.
32-4500 was used in western blot to investigate the HPV-16 splicing and its effect on primary human keratinocytes
|Li X,Johansson C,Cardoso Palacios C,Mossberg A,Dhanjal S,Bergvall M,Schwartz S||PloS one (8:null)||2013|
A synonymous polymorphic variation in ACADM exon 11 affects splicing efficiency and may affect fatty acid oxidation.
32-4500 was used in western blot to study a single-nucleotide polymorphism, rs278C to T, located far upstream of the MCAD gene.
|Bruun GH,Doktor TK,Andresen BS||Molecular genetics and metabolism (110:122)||2013|
A retroelement modifies pre-mRNA splicing: the murine Glrb(spa) allele is a splicing signal polymorphism amplified by long interspersed nuclear element insertion.
32-4500 was used in western blot to assess the effect of the Glrb(spa) LINE insertion on pre-mRNA splicing.
|Becker K,Braune M,Benderska N,Buratti E,Baralle F,Villmann C,Stamm S,Eulenburg V,Becker CM||The Journal of biological chemistry (287:31185)||2012|
Resveratrol, by modulating RNA processing factor levels, can influence the alternative splicing of pre-mRNAs.
32-4500 was used in western blot to report that resveratrol modulates alternative splicing in a target-specific manner.
|Markus MA,Marques FZ,Morris BJ||PloS one (6:null)||2011|
|Human||Not Cited||A conserved splicing mechanism of the LMNA gene controls premature aging.||Lopez-Mejia IC,Vautrot V,De Toledo M,Behm-Ansmant I,Bourgeois CF,Navarro CL,Osorio FG,Freije JM,Stévenin J,De Sandre-Giovannoli A,Lopez-Otin C,Lévy N,Branlant C,Tazi J||Human molecular genetics (20:4540)||2011|
|Mouse||Not Cited||RNA folding affects the recruitment of SR proteins by mouse and human polypurinic enhancer elements in the fibronectin EDA exon.||Buratti E,Muro AF,Giombi M,Gherbassi D,Iaconcig A,Baralle FE||Molecular and cellular biology (24:1387)||2004|
Identification by high-throughput imaging of the histone methyltransferase EHMT2 as an epigenetic regulator of VEGFA alternative splicing.
32-4500 was used in immunoprecipitation to study the function of histone methyltransferase EHMT2 in VEGFA alternative splicing
|Salton M,Voss TC,Misteli T||Nucleic acids research (42:13662)||2014|
||RNA folding affects the recruitment of SR proteins by mouse and human polypurinic enhancer elements in the fibronectin EDA exon.||Buratti E,Muro AF,Giombi M,Gherbassi D,Iaconcig A,Baralle FE||Molecular and cellular biology (24:1387)||2004|
|Human||Not Cited||Roles for SR proteins and hnRNP A1 in the regulation of c-src exon N1.||Rooke N,Markovtsov V,Cagavi E,Black DL||Molecular and cellular biology (23:1874)||2003|
|Rat||Not Cited||RNA splicing capability of live neuronal dendrites.||Glanzer J,Miyashiro KY,Sul JY,Barrett L,Belt B,Haydon P,Eberwine J||Proceedings of the National Academy of Sciences of the United States of America (102:16859)||2005|
alternative-splicing factor 1; arginine/serine-rich 1; P33 subunit; pre-mRNA-splicing factor SF2; pre-mRNA-splicing factor SF2, P33 subunit; SF2; SF2p33; SFRS1; splicing factor; splicing factor 2; splicing factor, arginine/serine-rich, 30-KD, A; SR splicing factor 1; SRp30a
ASF; OK/SW-cl.3; SF2; SF2P33; SFRS1; SRp30a; SRSF1