Immunofluorescence analysis of SFMBT2 in Hela and 293T Cells using Anti-SFMBT2 Recombinant Mouse Monoclonal Antibody (Cat. no. 730050). Cells were fixed with 3% Formaldehyde and lysed with 1% TritonX-100. Hela cells or 293T Cells were then single labeled with Anti-SFMBT2 Recombinant Mouse Monoclonal Antibody (Cat. no. 730050) and detected with Goat-anti-human Alexa Fluor dye F488 (green). FLAG cells were double labeled with human Fab and detected with Goat-anti-human Alexa Fluor dye F488 (green) as well as with Mouse-anti-FLAG-M2 and detected with Goat-anti-Mouse Alexa Fluor dye F647 (yellow). The DNA in the nucleus was detected with DAPI (Blue). Control means cells were labeled only with secondary antibody Alexa Fluor Dye.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||1-485 of 894 of SFMBT2 Protein.|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunoprecipitation (IP)||See 1 publications below|
The SFMBT2 (Scm-like with four MBT domains 2) gene shares high similarity with the Drosophila Scm (sex comb on midleg) gene. Sfmbt2 is a Polycomb group (PcG) gene that maps to the proximal region of Chromosome 2, and is a putative imprinted gene. Sfmbt2 is the first imprinted gene within this region to be identified. Studies indicate that six different translocations involving proximal chromosome 2 results in lethality when present as a maternal uniparental duplication.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Assessment of a method to characterize antibody selectivity and specificity for use in immunoprecipitation.
730050 was used in immunoprecipitation to describe a mass spectrometry-based method for scoring immunoprecipitation antibody quality
|Marcon E,Jain H,Bhattacharya A,Guo H,Phanse S,Pu S,Byram G,Collins BC,Dowdell E,Fenner M,Guo X,Hutchinson A,Kennedy JJ,Krastins B,Larsen B,Lin ZY,Lopez MF,Loppnau P,Miersch S,Nguyen T,Olsen JB,Paduch M,Ravichandran M,Seitova A,Vadali G,Vogelsang MS,Whiteaker JR,Zhong G,Zhong N,Zhao L,Aebersold R,Arrowsmith CH,Emili A,Frappier L,Gingras AC,Gstaiger M,Paulovich AG,Koide S,Kossiakoff AA,Sidhu SS,Wodak SJ,Gräslund S,Greenblatt JF,Edwards AM||Nature methods (12:725)||2015|