Western blot analysis of SFPQ in (lane 1) HeLa (lane 2) HepG2 (lane 3) A431 (lane 4) HUVEC (lane 5) NCI-H1299 (lane 6) WiDr (lane 7) Hepa 1-6 (lane 8) MM3MG lysates. Samples were separated on SDS-PAGE and probed with a SFPQ monoclonal antibody (Product # MA1-25325) at a dilution of 4 µg/ml. The antibody was developed using a Goat Anti-mouse IgG-Peroxidase and a chemiluminescent substrate.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Lysate of mouse bone marrow-derived stromal cell line.|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Radioimmune Assays (RIA)||Assay Dependent|
|Western Blot (WB)||2-4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-25325 detects SFPQ from human and mouse samples.
MA1-25325 has been successfully used in immunoprecipitation, radioimmune assay, immunocytochemistry, immunohistochemistry (paraffin) and Western blot procedures.
The MA1-25325 immunogen is lysate of mouse bone marrow-derived stromal cell line.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.