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Western blot analysis of SFPQ in (lane 1) HeLa (lane 2) HepG2 (lane 3) A431 (lane 4) HUVEC (lane 5) NCI-H1299 (lane 6) WiDr (lane 7) Hepa 1-6 (lane 8) MM3MG lysates. Samples were separated on SDS-PAGE and probed with a SFPQ monoclonal antibody (Product # MA1-25325) at a dilution of 4 µg/ml. The antibody was developed using a Goat Anti-mouse IgG-Peroxidase and a chemiluminescent substrate.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Lysate of mouse bone marrow-derived stromal cell line.|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Radioimmune Assays (RIA)||Assay Dependent|
|Western Blot (WB)||2-4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-25325 detects SFPQ from human and mouse samples.
MA1-25325 has been successfully used in immunoprecipitation, radioimmune assay, immunocytochemistry, immunohistochemistry (paraffin) and Western blot procedures.
The MA1-25325 immunogen is lysate of mouse bone marrow-derived stromal cell line.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
100 kDa DNA-pairing protein; DNA-binding p52/p100 complex, 100 kDa subunit; polypyrimidine tract binding protein associated; polypyrimidine tract-binding protein-associated splicing factor; polypyrimidine tract-binding protein-associated-splicing factor; POMP100; protein phosphatase 1, regulatory subunit 140; PSF; PTB-associated splicing factor; PTB-associated-splicing factor; splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated); splicing factor proline/glutamine rich (polypyrimidine tract-binding protein-associated); splicing factor proline/glutamine-rich; splicing factor, proline- and glutamine-rich
1110004P21Rik; 2810416M14Rik; 5730453G22Rik; 9030402K04Rik; AU021830; D4Ertd314e; Gm12940; OTTMUSG00000009329; POMP100; PPP1R140; PSF; REP1; SFPQ