Invitrogen

SFRP2 Monoclonal Antibody (OTI6C2)

View all (14) SFRP2 antibodies

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SFRP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human ovary tissue. To expose target proteins, 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a SFRP2 monoclonal antibody (Product # MA5-26881).

SFRP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded carcinoma of human liver tissue. To expose target proteins, 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a SFRP2 monoclonal antibody (Product # MA5-26881).

SFRP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded carcinoma of human lung tissue. To expose target proteins, 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a SFRP2 monoclonal antibody (Product # MA5-26881).

SFRP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded human kidney tissue. To expose target proteins, 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a SFRP2 monoclonal antibody (Product # MA5-26881).

SFRP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded human liver tissue. To expose target proteins, 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a SFRP2 monoclonal antibody (Product # MA5-26881).

SFRP2 Antibody in Western Blot (WB) — Western blot analysis of SFRP2 in 293T, HeLa, COLO205, human lung, human colon, human heart, mouse breast, mouse small intestine using 35 µg per lane. Samples were probed with SFRP2 (Product # MA5-26881) monoclonal antibody at a dilution of 1:500.

Product Details

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:150

-

Western Blot (WB)

1:500

-

Product Specifications

Species Reactivity

Human

Host / Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

OTI6C2

Immunogen

Human recombinant protein fragment corresponding to amino acids 25-295 of SFRP2 produced in E.coli

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2725338

Target Information

This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. Methylation of this gene is a potential marker for the presence of colorectal cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: FRP 2; FRP-2; MGC128911; MGC153618; MGC53344; SARP 1; SARP-1; SDF 5; secreted apoptosis related protein 1; Secreted apoptosis-related protein 1; Secreted frizzled-related protein 2; sFRP 2; sFRP-2; Sfrp2 protein; testicular tissue protein Li 170

Gene Aliases: FKSG12; FRP-2; FRP2; SARP1; SDF-5; SFRP2; UNQ361/PRO697

UniProt ID: (Human) Q96HF1

Entrez Gene ID: (Human) 6423

Molecular Function: G-protein coupled receptor receptor signaling molecule

patterning of blood vessels chondrocyte development outflow tract morphogenesis cardiac left ventricle morphogenesis apoptotic process G-protein coupled receptor signaling pathway cell-cell signaling response to nutrient positive regulation of cell proliferation negative regulation of cell proliferation male gonad development negative regulation of gene expression negative regulation of cardiac muscle cell apoptotic process negative regulation of epithelial to mesenchymal transition positive regulation of endopeptidase activity negative regulation of Wnt signaling pathway collagen fibril organization positive regulation of cell growth negative regulation of cell growth negative regulation of cell migration negative regulation of BMP signaling pathway cellular response to extracellular stimulus positive regulation of peptidyl-serine phosphorylation positive regulation of cell adhesion mediated by integrin positive regulation of catenin import into nucleus non-canonical Wnt signaling pathway post-anal tail morphogenesis response to drug negative regulation of mesodermal cell fate specification embryonic digit morphogenesis positive regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process negative regulation of JUN kinase activity positive regulation of fat cell differentiation positive regulation of osteoblast differentiation positive regulation of angiogenesis negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter digestive tract morphogenesis negative regulation of epithelial cell proliferation negative regulation of peptidyl-tyrosine phosphorylation convergent extension involved in axis elongation bone morphogenesis sclerotome development negative regulation of dermatome development hematopoietic stem cell proliferation cellular response to X-ray negative regulation of canonical Wnt signaling pathway planar cell polarity pathway involved in neural tube closure Wnt signaling pathway involved in somitogenesis positive regulation of canonical Wnt signaling pathway negative regulation of extrinsic apoptotic signaling pathway via death domain receptors negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage regulation of dopaminergic neuron differentiation midbrain morphogenesis regulation of stem cell division negative regulation of planar cell polarity pathway involved in axis elongation

Process(es)

fibronectin binding G-protein coupled receptor activity integrin binding Wnt-protein binding Wnt-activated receptor activity receptor agonist activity endopeptidase activator activity