Invitrogen

SFRP2 Monoclonal Antibody (OTI6C2)

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SFRP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human ovary tissue. To expose target proteins, 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a SFRP2 monoclonal antibody (Product # MA5-26881).

SFRP2 Antibody in Western Blot (WB) — Western blot analysis of SFRP2 in 293T, HeLa, COLO205, human lung, human colon, human heart, mouse breast, mouse small intestine using 35 µg per lane. Samples were probed with SFRP2 (Product # MA5-26881) monoclonal antibody at a dilution of 1:500.

Product Details

Tested Applications

Dilution

Immunohistochemistry (Paraffin) (IHC (P))

1:150

Western Blot (WB)

1:500

Product Specifications

Species Reactivity

Human

Host / Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

OTI6C2

Immunogen

Human recombinant protein fragment corresponding to amino acids 25-295 of SFRP2 produced in E.coli

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2725338

Target Information

The frizzled gene, originally identified in Drosophila melanogaster, was shown to be involved in the development of tissue polarity. The mammalian homolog of frizzled, as well as several secreted, mammalian, frizzled-related proteins such as FRP-1 (also designated SARP2), FRP-2 (also designated SARP1), FRP-3, FRP-4 and SARP3 (also designated FRP-5), have been identified. The frizzled proteins contain seven transmembrane domains and a cysteine-rich domain in the extra carboxy-terminal Ser/Thr-xxx-Val motif, and they function as receptors for Wnt. The frizzled-1 gene maps to human chromosome 7q21 and is expressed in adult heart, placenta, lung, kidney, pancreas, prostate and ovary, as well as in fetal lung and kidney. Frizzled-2 is expressed in adult heart and fetal brain, lung and kidney. The frizzled-related proteins FRP-1, FRP-2, FRP-3, FRP-4 and SARP3 are secreted proteins that contain regions of homology to the cysteine-rich, ligand-binding domain of frizzled and a conserved, hydrophilic carboxy-terminus. The gene encoding human SARP3 maps to chromosome 4q31.3 and is expressed in retinal pigment epithelium (RPE) and pancreas, while expression of FRP-1, 2 and 4 is high in developing tissues. The FRPs/SARPs are involved in the Wnt signaling pathway by regulating the intracellular levels of beta-catenin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Frizzled related protein 2; FRP 2; FRP-2; FRP2; MGC128911; MGC153618; MGC53344; SARP 1; SARP-1; SARP1; SDF 5; SDF5; Secreted apoptosis related protein 1; Secreted apoptosis-related protein 1; Secreted frizzled related protein 2; Secreted frizzled-related protein 2; sFRP 2; sFRP-2; SFRP2; Sfrp2 protein; testicular tissue protein Li 170

Gene Aliases: FKSG12; FRP-2; FRP2; SARP1; SDF-5; SFRP2; UNQ361/PRO697

UniProt ID: (Human) Q96HF1

Entrez Gene ID: (Human) 6423

Molecular Function: G-protein coupled receptor receptor signaling molecule