HYB 245-01-02 is specific for human SP-D.
HYB 245-01-02 has been successfully used in Immunohistochemistry, ELISA and Western blot procedures. In immunohistochemical staining on paraffin embedded tissues, HYB 245-01 reacts specifically with SP-D.
Immunoreactivity can be found in epithelial cells of the lung, skin, small intestine, esophagus, gall-bladder, kidney and urinary bladder. In Western blotting after SDS-PAGE HYB 245-01 reacts strongly with SP-D, both in reduced and non-reduced forms. HYB 245-01 can be used for ELISA, but generally gives a weak signal.
The HYB 245-01-02 immunogen is recombinant neck CRD SP-D.
NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL
Surfactant protein D (SP-D) is synthesized and secreted by lung epithelial cells. It belongs to group III of the family of C-type lectins and members of this group has overall structure consisting of multiple globular 'head' regions linked by triple-helical, collagen-like, strands. This group also includes SP-A and the serum proteins mannan-binding protein, conglutinin and collectin-43, all of which have been shown to bind to the C1q receptor found on a wide variety of cells. Both SP-D and SP-A have been shown to enhance oxygen radical production by alveolar macrophages. The serum concentration is 88 ng/mL in healthy individuals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: COLEC7; Collectin-7; Collectin7; Lung surfactant protein D; PSP-D; Pulmonary surfactant apoprotein; Pulmonary surfactant-associated protein D; SFTP4; SFTPD; SP-D; Surfactant associated prote; surfactant-associated protein, pulmonary 4
Gene Aliases: COLEC7; PSP-D; PSPD; SFTP4; SFTPD; SP-D
UniProt ID: (Human) P35247
Entrez Gene ID: (Human) 6441