Proteintech

SGK1 Polyclonal Antibody

Product Image Gallery

SGK1 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of HEK-293 cells using 23394-1-AP (SGK1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

SGK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer slide using 23394-1-AP (SGK1 Antibody) at dilution of 1:50.

SGK1 Antibody in Flow Cytometry (Flow) — 1X10^6 HeLa cells were stained with 0.2ug SGK1 antibody (23394-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

SGK1 Antibody in Immunoprecipitation (IP) — IP result of anti-SGK1 (IP:23394-1-AP, 4ug; Detection:23394-1-AP 1:600) with HEK-293 cells lysate 1480ug.

Product Details

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunocytochemistry (ICC)

1:10-1:100

Immunofluorescence (IF)

1:10-1:100

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunoprecipitation (IP)

0.5-4.0 µg

Western Blot (WB)

View 1 publication 1 publication

Product Specifications

Species

Human, Mouse

Published species

Human

Expression System

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SGK1 Fusion Protein Ag17888

Conjugate

Unconjugated

Form

Liquid

Concentration

0.41 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

This antibody is specific to SGK1.

Target Information

Serum and glucocorticoid-regulated protein kinase 1 (SGK1) is a member of the AGC family of serine/threonine kinases that plays an important role in cellular stress response and cell survival. SGK1 is transcriptionally activated by numerous stimuli, including serum, glucocorticoids, osmotic stress, and hormones. SGK1 functions as a regulator of cell survival and ion channels and transporters such as ENaC or KCNA3/Kv1. 3. It phosphorylates and negatively regulates pro-apoptotic FOXO3A. SGK1 is thought to play an essential function in nephropathy and cardiovascular development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: serine/threonine protein kinase SGK; Serine/threonine-protein kinase Sgk1; serum and glucocorticoid-dependent protein kinase; Serum/glucocorticoid-regulated kinase 1; Sgk1 variant i3

Gene Aliases: SGK; SGK1

UniProt ID: (Human) O00141, (Mouse) Q9WVC6

Entrez Gene ID: (Human) 6446, (Mouse) 20393

Molecular Function: annexin calcium-binding protein calmodulin intracellular calcium-sensing protein kinase non-receptor serine/threonine protein kinase protein kinase transfer/carrier protein transferase