Proteintech

SGK1 Polyclonal Antibody

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SGK1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HEK-293 cells using 23394-1-AP (SGK1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

SGK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer slide using 23394-1-AP (SGK1 Antibody) at dilution of 1:50.

SGK1 Antibody in Flow Cytometry (Flow) — 1X10^6 HeLa cells were stained with 0.2ug SGK1 antibody (23394-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

SGK1 Antibody in Immunoprecipitation (IP) — IP result of anti-SGK1 (IP:23394-1-AP, 4ug; Detection:23394-1-AP 1:600) with HEK-293 cells lysate 1480ug.

Product Details

23394-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:10-1:100	-
Flow Cytometry (Flow)	0.2 µg/1x10^6 cells	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SGK1 Fusion Protein Ag17888 (1-431 aa encoded by BC001263) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.41 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

This antibody is specific to SGK1.

Immunogen sequence: MTVKTEAAK GTLTYSRMRG MVAILIAFMK QRRMGLNDFI QKIANNSYAC KHPEVQSILK ISQPQEPELM NANPSPPPSP SQQINLGPSS NPHAKPSDFH FLKVIGKGSF GKVLLARHKA EEVFYAVKVL QKKAILKKKE EKHIMSERNV LLKNVKHPFL VGLHFSFQTA DKLYFVLDYI NGGELFYHLQ RERCFLEPRA RFYAAEIASA LGYLHSLNIV YRDLKPENIL LDSQGHIVLT DFGLCKENIE HNSTTSTFCG TPEYLAPEVL HKQPYDRTVD WWCLGAVLYE MLYGLPPFYS RNTAEMYDNI LNKPLQLKPN ITNSARHLLE GLLQKDRTKR LGAKDDFMEI KSHVFFSLIN WDDLINKKIT PPFNPNVSGP NDLRHFDPEF TEEPVPNSIG KSPDSVLVTA SVKEAAEAFL GFSYAPPTDS FL (1-431 aa encoded by BC001263)

Target Information

Serum and glucocorticoid-regulated protein kinase 1 (SGK1) is a member of the AGC family of serine/threonine kinases that plays an important role in cellular stress response and cell survival. SGK1 is transcriptionally activated by numerous stimuli, including serum, glucocorticoids, osmotic stress, and hormones. SGK1 functions as a regulator of cell survival and ion channels and transporters such as ENaC or KCNA3/Kv1. 3. It phosphorylates and negatively regulates pro-apoptotic FOXO3A. SGK1 is thought to play an essential function in nephropathy and cardiovascular development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Inhibition of serum- and glucocorticoid-inducible kinase 1 enhances TLR-mediated inflammation and promotes endotoxin-driven organ failure.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Zhou H,Gao S,Duan X,Liang S,Scott DA,Lamont RJ,Wang H

Tue Sep 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: serine/threonine protein kinase SGK; Serine/threonine-protein kinase Sgk1; serum and glucocorticoid-dependent protein kinase; Serum/glucocorticoid-regulated kinase 1; Sgk1 variant i3

Gene Aliases: SGK; SGK1

UniProt ID: (Human) O00141, (Mouse) Q9WVC6

Entrez Gene ID: (Human) 6446, (Mouse) 20393

Molecular Function: protein modifying enzyme