|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the C-terminal residues S(1951) G P I Y P G L F D I R S S(1964) of rat Shank1a.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||1:100 - 1:200|
|Western Blot (WB)||1:500 - 1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-4180 detects SHANK1a in human, rat, and mouse samples.
PA1-4180 has been successfully used in Western blot, immunofluorescence, and immunofluoresence procedures. By Western blot this antibody detects a ~212 kDa protein representing SHANK1a in rat brain lysate.
The PA1-4180 immunogen is a synthetic peptide corresponding to the C-terminal residues S(1951) G P I Y P G L F D I R S S(1964) of rat Shank1a.
Maintain at 4°C for 3 months or at -20°C for longer periods. Avoid freeze/thaw cycles.
Shank is a novel family of postsynaptic density proteins (PSD) that bind to PSD-95-associated protein GKAP. Its putative function is to act as a scaffold protein in the PSD to cross-link NMDA receptors/PSD-95 complexes and couple them to regulators of the actin cytoskeleton. Shank1a is a splice variant of Shank1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.