Invitrogen

SHC3 Polyclonal Antibody

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SHC3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of SHC3 in paraffin-embedded Human Stomach cancer tissue. Samples were incubated with polyclonal antibody (Product # PA5-119411) at a dilution of 10 µg/mL, followed by HRP-Linked Caprine Anti-Rabbit IgG using a dilution of 2 µg/mL.

SHC3 Antibody in Western Blot (WB) — Western blot analysis of SHC3 in Lane 1: Wild-type HepG2 cell lysate; Lane 2: SHC3 knockout HepG2 cell lysate . Samples were incubated with polyclonal antibody (Product # PA5-119411) at a dilution of 3 µg/mL, followed by HRP-Linked Caprine Anti-Rabbit IgG using a dilution of 0.2 µg/mL.

Product Details

PA5-119411

Applications	Tested Dilution	Publications
Western Blot (WB)	0.5-2 µg/mL	-
Immunohistochemistry (Paraffin) (IHC (P))	5-20 µg/mL	-
Immunocytochemistry (ICC/IF)	5-20 µg/mL	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein SHC-Transforming Protein 3
Conjugate	Unconjugated
Form	Liquid
Concentration	500 µg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS with 50% glycerol
Contains	0.05% Proclin 300
Storage conditions	-20°C

Target Information

Signaling adapter that couples activated growth factor receptors to signaling pathway in neurons. Involved in the signal transduction pathways of neurotrophin-activated Trk receptors in cortical neurons.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: N-Shc; Neuronal Shc; Protein Rai; SH2 domain protein C3; SHC (Src homology 2 domain containing) transforming protein 3; SHC-transforming protein 3; SHC-transforming protein C; Shc3 p51; src homology 2 domain-containing transforming protein C3; Src homology 2 domain-containing-transforming protein C3

Gene Aliases: N-Shc; NSHC; RAI; SHC3; SHCC

UniProt ID: (Human) Q5T7I7

Entrez Gene ID: (Human) 53358

Molecular Function: scaffold/adaptor protein