Immunofluorescence analysis of SHP-1 was performed using 70% confluent log phase Jurkat cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SHP-1 Mouse Monoclonal Antibody (MA5-11669) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear and cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Mouse, Human|
|Host / Isotype||Mouse / IgG2b, kappa|
|Immunogen||Recombinant human SHP1 protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Western Blot (WB)||0.5-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 4 publications below|
MA5-11669 targets SHP-1 in WB applications and shows reactivity with Human samples.
The MA5-11669 immunogen is recombinant human SHP1 protein.
Shp-1 is a SH2-containing protein-tyrosine phosphatase 1 (SHP-1). SHP-1 is known to play a crucial role in the regulation of hematopoiesis and it regulates the transcriptional activity stimulated by the erythropoietin (EPO)-induced JAK/STAT and MAPK pathways and is involved in the signaling machinery responsible for erythroid differentiation and suppression of apoptosis. Furthermore, the observation that SHP-1 associates with RAFTK/Pyk2/CAK activated by beta-chemokine receptor (CCR5) and SHP-1 also associates with the adaptor protein Grb2 and the Src-related kinase Syk indicates the role of SHP-1 in modulating cell migration, proliferation, and immune functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The protein tyrosine phosphatase SHP-1 modulates the suppressive activity of regulatory T cells.
MA5-11669 was used in western blot to study the role of protein tyrosine phosphatase SHP-1 in modulating the suppressive activity of regulatory T cells
|Iype T,Sankarshanan M,Mauldin IS,Mullins DW,Lorenz U||Journal of immunology (Baltimore, Md. : 1950) (185:6115)||2010|
Integrative analysis of epigenetic modulation in melanoma cell response to decitabine: clinical implications.
MA5-11669 was used in western blot to study the resistence of cancers against decitabine treatment and its mechanism
|Halaban R,Krauthammer M,Pelizzola M,Cheng E,Kovacs D,Sznol M,Ariyan S,Narayan D,Bacchiocchi A,Molinaro A,Kluger Y,Deng M,Tran N,Zhang W,Picardo M,Enghild JJ||PloS one (4:null)||2009|
Deficiency of the Src homology region 2 domain-containing phosphatase 1 (SHP-1) causes enrichment of CD4+CD25+ regulatory T cells.
MA5-11669 was used in western blot to study the role of SHP-1 phosphatase deficiency in the enrichment of CD4+CD25+ regulatory T cells
|Carter JD,Calabrese GM,Naganuma M,Lorenz U||Journal of immunology (Baltimore, Md. : 1950) (174:6627)||2005|
Localization of Src homology 2 domain-containing phosphatase 1 (SHP-1) to lipid rafts in T lymphocytes: functional implications and a role for the SHP-1 carboxyl terminus.
MA5-11669 was used in western blot to study the localization of Src homology 2 domain-containing phosphatase 1 to lipid rafts in T lymphocytes
|Fawcett VC,Lorenz U||Journal of immunology (Baltimore, Md. : 1950) (174:2849)||2005|