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This is specific for human SIAH 1 and 2 and Drosophila SINA in proliferating cells and stem cells. This does not detect Drosophila SINAH or normal, nondividing cells.
SIAH, the human homologue of the Drosophila seven in absentia (sina) gene, is a tumor suppressor protein that is expressed in intestinal epithelium and activated during apoptosis. Human SIAH proteins are produced as two distinct gene products, SIAH-1, and a slightly larger protein SIAH-2, which share a highly conserved C-terminal sequence and differ in their N-terminal regions. SIAH-1 is a 32 kDa protein that contains an N-terminal RING-finger domain, which is required for proteolysis, and a cystein-rich C-terminal domain, which regulates oligomerization and SIAH binding to target proteins. As a tumor suppressor, SIAH-1 binds DCC (deleted in colorectal cancer) and regulates DCC degradation via the ubiquitin-proteasome pathway. SIAH-1 also binds a Bcl-2 related protein, Bag-1, thereby inhibiting cell growth. The majority of SIAH-1 is localized to the nucleus, however a small percentage is detected in the cytoplasm. This nuclear localization suggests that SIAH proteins may interact with other nuclear matrix proteins and DNA.
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Protein Aliases: anon-fast-evolving-2H7; CG9949-PA; CG9949-PB; CG9949-PC; E3 ubiquitin-protein ligase SIAH1; E3 ubiquitin-protein ligase SIAH2; E3 ubiquitin-protein ligase sina; EC 6.3.2; FLJ08065; HL08111p; hSIAH1; RING-type E3 ubiquitin transferase SIAH1; RING-type E3 ubiquitin transferase SIAH2; RING-type E3 ubiquitin transferase sina; seven in absenti; seven in absentia 1A; seven in absentia 2; Seven in absentia homolog 1; Seven in absentia homolog 2; Seven in absentia protein; seven-in-absentia; Siah-1; Siah-1a; Siah-2; sina-PA; sina-PB; sina-PC
Gene Aliases: anon-EST:fe2H7; CG9949; Dmel\CG9949; Dmel_CG9949; hSiah2; HUMSIAH; id:ibd2635; sia; SIAH; SIAH1; SIAH1A; SIAH2; SINA; zgc:56026