Invitrogen

SIN3A Recombinant Rabbit Monoclonal Antibody (JA94-31)

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Datasheet

Protocols

Questions & Answers

Tech Support

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SIN3A Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemical analysis of SIN3A in NIH-3T3 cells using a SIN3A Monoclonal antibody (Product # MA5-32720) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

SIN3A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of SIN3A of paraffin-embedded Human kidney tissue using a SIN3A Monoclonal antibody (Product #MA5-32720). Counter stained with hematoxylin.

SIN3A Antibody in Western Blot (WB) — Western blot analysis of SIN3A in 293T cell lysate using a SIN3A Monoclonal antibody (Product # MA5-32720) at a dilution of 1:1,000.

SIN3A Antibody in Flow Cytometry (Flow) — Flow Cytometric analysis of SIN3A in MCF-7 cells using a SIN3A Monoclonal Antibody (Product # MA5-32720) at a dilution of 1:100, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black).

Product Details

MA5-32720

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (IHC)	1:50	-
Immunocytochemistry (ICC/IF)	1:50-1:200	-
Flow Cytometry (Flow)	1:50-1:100	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Recombinant Monoclonal
Type	Antibody
Clone	JA94-31
Immunogen	Synthetic peptide within Human mSin3A aa 1224-1273 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains	0.05% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2809997

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Mammalian Sin3 (mSin3) is a 150 kDa protein closely related to the yeast SIN3 repressor protein involved in the transcriptional repression of many genes. There are two mammalian isoforms, mSin3A and mSin3B. Containing 4 paired amphipathic helix domains (PAH domains), mSin3A and mSin3B have been shown to directly interact with several other transcriptional repressor proteins including HDAC1, HDAC2, RbAp46, the methyl CpG binding protein MeCP2, the Mad/Max heterodimer, and the corepressors silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) and nuclear receptor corepressor (N-CoR).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Histone deacetylase complex subunit Sin3a; Mammalian Sin3A; Paired amphipathic helix protein Sin3a; SIN; SIN3 homolog A, transcription regulator; SIN3 transcription regulator homolog A; SIN3A; transcriptional co-repressor Sin3A; Transcriptional corepressor Sin3a; transcriptional regulator, SIN3 yeast homolog A; transcriptional regulator, SIN3A

Gene Aliases: AW553200; Kiaa4126; mKIAA4126; mSin3A; Sin3; SIN3A

UniProt ID: (Human) Q96ST3, (Mouse) Q60520

Entrez Gene ID: (Human) 25942, (Rat) 363067, (Mouse) 20466

Molecular Function: chromatin/chromatin-binding, or -regulatory protein