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SIN3A Antibody (MA5-32720) in ICC/IF
Immunocytochemical analysis of SIN3A in NIH-3T3 cells using a SIN3A Monoclonal antibody (Product # MA5-32720) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Product Details
MA5-32720
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JA94-31
Immunogen
Synthetic peptide within Human mSin3A aa 1224-1273
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2809997
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Mammalian Sin3 (mSin3) is a 150 kDa protein closely related to the yeast SIN3 repressor protein involved in the transcriptional repression of many genes. There are two mammalian isoforms, mSin3A and mSin3B. Containing 4 paired amphipathic helix domains (PAH domains), mSin3A and mSin3B have been shown to directly interact with several other transcriptional repressor proteins including HDAC1, HDAC2, RbAp46, the methyl CpG binding protein MeCP2, the Mad/Max heterodimer, and the corepressors silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) and nuclear receptor corepressor (N-CoR).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Histone deacetylase complex subunit Sin3a; Mammalian Sin3A; Paired amphipathic helix protein Sin3a; SIN; SIN3 homolog A, transcription regulator; SIN3 transcription regulator homolog A; SIN3A; transcriptional co-repressor Sin3A; Transcriptional corepressor Sin3a; transcriptional regulator, SIN3 yeast homolog A; transcriptional regulator, SIN3A
Gene Aliases: AW553200; Kiaa4126; mKIAA4126; mSin3A; Sin3; SIN3A
UniProt ID: (Human) Q96ST3, (Mouse) Q60520
Entrez Gene ID: (Human) 25942, (Rat) 363067, (Mouse) 20466
transcription regulatory region sequence-specific DNA binding
RNA polymerase II activating transcription factor binding
RNA polymerase II repressing transcription factor binding
RNA polymerase II transcription corepressor activity
chromatin binding
transcription factor activity, sequence-specific DNA binding
RNA binding
histone deacetylase activity
protein binding
protein complex binding
protein deacetylase activity
DNA binding
transcription corepressor activity
transcription factor binding
chromatin/chromatin-binding, or -regulatory protein
negative regulation of transcription from RNA polymerase II promoter
in utero embryonic development
activation of innate immune response
positive regulation of defense response to virus by host
hematopoietic progenitor cell differentiation
DNA replication
transcription, DNA-templated
protein deacetylation
aging
regulation of hormone levels
positive regulation of G2/M transition of mitotic cell cycle
histone deacetylation
positive regulation of chromatin silencing
cellular protein localization
negative regulation of circadian rhythm
negative regulation of apoptotic process
regulation of transcription from RNA polymerase II promoter in response to oxidative stress
cellular lipid metabolic process
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
rhythmic process
response to methylglyoxal
cellular response to glucose stimulus
negative regulation of protein localization to nucleus
negative regulation of histone H3-K27 acetylation
cellular response to dopamine
negative regulation of transcription regulatory region DNA binding
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
response to organonitrogen compound
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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