|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (328 RSGGQRHSPLSQRHSPAPQC 347) of SINTBAD/TBKBP1 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||5 µg/ml|
|Western Blot (WB)||0.5-4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
SINTBAD is an adaptor protein belonging to the fibrillar collagen family, consisting of trimers of identical alpha 1 chains which are linked to each other by interchain disulfide bonds. It is essential for signal transduction during viral infection and plays a major role in the NF-kB pathway. SINTBAD is thus required for efficient IRF (interferon regulatory factor) activation by Sendai virus. The protein binds TBK1 (TANK-binding kinase) and IKKi (inhibitor of nuclear factor B kinase, inducible) via a newly identified TBK1/IKKi-binding domain (TBD), which it shares with TANK and NAP1. Binding to kinases via TBD is essential for IRF3 (interferon regulatory factor 3) activation which controls the expression of antiviral genes during infection. The protein has a ubiquitous expression with highest levels in ovary, followed by testis and brain. Human SINTBAD gene is mapped to chromosome 17q21.32.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.