|Flow Cytometry (Flow)||1.0 ug/10^6 cells|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Tested Species reactivity||Rat|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Rat Peritoneal Macrophages|
|Storage buffer||PBS with 4-5mg/ml BSA|
|Contains||0.02% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
MA5-17501 targets SIRP in FACS and IHC (F) applications and shows reactivity with Rat samples.
The MA5-17501 immunogen is rat Peritoneal Macrophages.
CD172a, also known as signal-regulatory protein alpha, is a receptor-type transmembrane glycoprotein expressed on cells of myeloid origin, including granulocytes, dendritic cells (DCs), macrophages, mast cells and haematopoietic stem cells. CD172a acts as a substrate for several activated tyrosine kinases, including EGFR, PDGFR, src and insulin receptor and is involved in the negative regulation of receptor tyrosine kinase-coupled signaling pathways. Ligand binding of CD172a to integrin-associated protein CD47, results in tyrosine kinase phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within the cytoplasmic region of CD172a, mediating the recruitment and activation of the tyrosine phosphatases SHP-1 and SHP-2. These then act as regulators of cellular function, through dephosphorylation of specific substrates. Ligation of CD172a with CD47 has been demonstrated in several regulatory processes, including the inhibition of host cell phagocytosis by macrophages and the bi-directional activation of T cells and DCs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: BIT; Brain Ig-like molecule with tyrosine-based activation motifs; Brain immunoglobulin like protein with tyrosine - based activation motifs; CD172 antigen-like family member A; CD172A; Inhibitory receptor SHPS-1; Macrophage fusion receptor; Macrophage membrane protein MFP150; MYD-1; P84; Protein tyrosine phosphatase non-receptor type substrate 1 (SHP substrate 1); Protein tyrosine phosphatase, non-receptor type substrate 1 (SHP substrate 1); PTPNS1; SHP substrate 1; SHPS-1; SHPS1; Signal-regulatory protein alpha-1; SIRP-ALPHA-1; SIRPalpha; SIRPalpha2; Tyrosine-protein phosphatase non-receptor type substrate 1
Gene Aliases: Bit; Mfr; Ptpns1; SHPS-1; Shps1; Sirp; Sirpa
UniProt ID: (Rat) P97710
Entrez Gene ID: (Rat) 25528
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