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Human HDFn cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). The cells were incubated with the antibody at 0.5 µg/mL overnight at 4°C. Secondary antibody: (Red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue).
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant human SIRT1, 2, 3, 4.|
|Storage buffer||HEPES buffered saline|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunocytochemistry (ICC)||1.0 ug/ml|
|Western Blot (WB)||1-2 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The Silent Information Regulator (SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and have important functions in the regulation of metabolism, growth and differentiation, inflammation, cellular survival, as well as in senescence and lifespan extension. Sirtuins, including SIRT1-7, are human homologs of yeast Sir2p. Sirtuins are NAD+-dependent histone/protein deacetylases (HDAC) which regulate cellular metabolism, e.g. energy metabolism, and thereby are associated with aging and several age-related diseases. SIRT1 has the closest homology to the yeast Sir2p and is widely expressed in fetal and adult tissues. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically via lysine residue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
EC 3.5.1.-; hSIR2; mSIR2a; NAD-dependent deacetylase sirtuin 1; NAD-dependent deacetylase sirtuin-1; NAD-dependent protein deacetylase sirtuin-1; regulatory protein SIR2 homolog 1; SIR1; sir2-like 1; SIR2-like protein 1; SIR2L1; sirtuin 1; sirtuin 1 ((silent mating type information regulation 2, homolog) 1; sirtuin type 1
AA673258; SIR2; Sir2a; SIR2alpha; SIR2L1; SIRT1