Proteintech

SIRT4 Monoclonal Antibody (1D5A2)

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Antibody Testing Data (4)

FIGURE: 1 / 4

SIRT4 Antibody (66543-1-IG) in WB

NCCIT cell were subjected to SDS PAGE followed by western blot with 66543-1-Ig (SIRT4 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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Product Details

66543-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:20,000-1:80,000

View 19 publications 19 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Pig, Rat

Published species

Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1D5A2

Immunogen

SIRT4 Fusion Protein Ag17347 (1-314 aa encoded by BC109319)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: MKMSFALTF RSAKGRWIAN PSQPCSKASI GLFVPASPPL DPEKVKELQR FITLSKRLLV MTGAGISTES GIPDYRSEKV GLYARTDRRP IQHGDFVRSA PIRQRYWARN FVGWPQFSSH QPNPAHWALS TWEKLGKLYW LVTQNVDALH TKAGSRRLTE LHGCMDRVLC LDCGEQTPRG VLQERFQVLN PTWSAEAHGL APDGDVFLSE EQVRSFQVPT CVQCGGHLKP DVVFFGDTVN PDKVDFVHKR VKEADSLLVV GSSLQVYSGY RFILTAWEKK LPIAILNIGP TRSDDLACLK LNSRCGELLP LIDPC (1-314 aa encoded by BC109319)

Target Information

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Amino acids downregulate SIRT4 to detoxify ammonia through the urea cycle.

Nature metabolism

Hu SH,Feng YY,Yang YX,Ma HD,Zhou SX,Qiao YN,Zhang KH,Zhang L,Huang L,Yuan YY,Lin Y,Zhang XY,Li Y,Li HT,Zhao JY,Xu W,Zhao SM

Sat Apr 01 00:00:00 EDT 2023

Korean Red Ginseng-Induced SIRT3 Promotes the Tom22-HIF-1α Circuit in Normoxic Astrocytes.

Cells

Kim H,Moon S,Lee D,Park J,Kim CH,Kim YM,Choi YK

Tue May 30 00:00:00 EDT 2023

Metrnl Alleviates Lipid Accumulation by Modulating Mitochondrial Homeostasis in Diabetic Nephropathy.

Diabetes

Zhou Y,Liu L,Jin B,Wu Y,Xu L,Chang X,Hu L,Wang G,Huang Y,Song L,Zhang T,Wang Y,Xiao Y,Zhang F,Shi M,Liu L,Wang T,Yan R,Guo B

Mon May 01 00:00:00 EDT 2023

miR-124-3p improves mitochondrial function of renal tubular epithelial cells in db/db mice.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Liang L,Wo C,Yuan Y,Cao H,Tan W,Zhou X,Wang D,Chen R,Shi M,Zhang F,Xiao Y,Liu L,Zhou Y,Zhang T,Wang Y,Guo B

Wed Mar 01 00:00:00 EST 2023

miR-124-3p improves mitochondrial function of renal tubular epithelial cells in db/db mice.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Liang L,Wo C,Yuan Y,Cao H,Tan W,Zhou X,Wang D,Chen R,Shi M,Zhang F,Xiao Y,Liu L,Zhou Y,Zhang T,Wang Y,Guo B

Wed Mar 01 00:00:00 EST 2023

AGER1 deficiency-triggered ferroptosis drives fibrosis progression in nonalcoholic steatohepatitis with type 2 diabetes mellitus.

Cell death discovery

Gong Y,Liu Z,Zhang Y,Zhang J,Zheng Y,Wu Z

Tue Jun 06 00:00:00 EDT 2023

Proteomic profiling reveals the potential mechanisms and regulatory targets of sirtuin 4 in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced Parkinson's mouse model.

Frontiers in neuroscience

Weng H,Song W,Fu K,Guan Y,Cai G,Huang E,Chen X,Zou H,Ye Q

Sat Feb 11 00:00:00 EST 2023

Novel Thermosensitive Hydrogel Promotes Spinal Cord Repair by Regulating Mitochondrial Function.

ACS applied materials & interfaces

Li Y,Yang L,Hu F,Xu J,Ye J,Liu S,Wang L,Zhuo M,Ran B,Zhang H,Ye J,Xiao J

Wed Jun 08 00:00:00 EDT 2022

Novel Thermosensitive Hydrogel Promotes Spinal Cord Repair by Regulating Mitochondrial Function.

ACS applied materials & interfaces

Li Y,Yang L,Hu F,Xu J,Ye J,Liu S,Wang L,Zhuo M,Ran B,Zhang H,Ye J,Xiao J

Wed Jun 08 00:00:00 EDT 2022

Novel Thermosensitive Hydrogel Promotes Spinal Cord Repair by Regulating Mitochondrial Function.

ACS applied materials & interfaces

Li Y,Yang L,Hu F,Xu J,Ye J,Liu S,Wang L,Zhuo M,Ran B,Zhang H,Ye J,Xiao J

Wed Jun 08 00:00:00 EDT 2022

Novel Thermosensitive Hydrogel Promotes Spinal Cord Repair by Regulating Mitochondrial Function.

ACS applied materials & interfaces

Li Y,Yang L,Hu F,Xu J,Ye J,Liu S,Wang L,Zhuo M,Ran B,Zhang H,Ye J,Xiao J

Wed Jun 08 00:00:00 EDT 2022

Correlation between the reduction in hippocampal SirT2 expression and depressive-like behaviors and neurological abnormalities in olfactory bulbectomized mice.

Neuroscience research

Takahashi K,Kurokawa K,Hong L,Miyagawa K,Mochida-Saito A,Takeda H,Tsuji M

Thu Sep 01 00:00:00 EDT 2022

Omentin1 ameliorates myocardial ischemia-induced heart failure via SIRT3/FOXO3a-dependent mitochondrial dynamical homeostasis and mitophagy.

Journal of translational medicine

Hu J,Liu T,Fu F,Cui Z,Lai Q,Zhang Y,Yu B,Liu F,Kou J,Li F

Tue Oct 04 00:00:00 EDT 2022

Rosmarinic Acid Inhibits Mitochondrial Damage by Alleviating Unfolded Protein Response.

Frontiers in pharmacology

Cai G,Lin F,Wu D,Lin C,Chen H,Wei Y,Weng H,Chen Z,Wu M,Huang E,Ye Z,Ye Q

Sat Jul 16 00:00:00 EDT 2022

20-HETE synthesis inhibition attenuates traumatic brain injury-induced mitochondrial dysfunction and neuronal apoptosis via the SIRT1/PGC-1α pathway: A translational study.

Cell proliferation

Cui W,Wu X,Shi Y,Guo W,Luo J,Liu H,Zheng L,Du Y,Wang P,Wang Q,Feng D,Ge S,Qu Y

Mon Feb 01 00:00:00 EST 2021

SETD8 potentiates constitutive ERK1/2 activation via epigenetically silencing DUSP10 expression in pancreatic cancer.

Cancer letters

Liu M,Qin Y,Hu Q,Liu W,Ji S,Xu W,Fan G,Ye Z,Zhang Z,Xu X,Yu X,Zhuo Q

Sun Feb 28 00:00:00 EST 2021

SIRT1 coordinates with the CRL4B complex to regulate pancreatic cancer stem cells to promote tumorigenesis.

Cell death and differentiation

Leng S,Huang W,Chen Y,Yang Y,Feng D,Liu W,Gao T,Ren Y,Huo M,Zhang J,Yang Y,Wang Y

Wed Dec 01 00:00:00 EST 2021

SIRT4 is the molecular switch mediating cellular proliferation in colorectal cancer through GLS mediated activation of AKT/GSK3β/CyclinD1 pathway.

Carcinogenesis

Cui Y,Bai Y,Yang J,Yao Y,Zhang C,Liu C,Shi J,Li Q,Zhang J,Lu X,Zhang Y

Sat Apr 17 00:00:00 EDT 2021

Influence of psychostimulants and opioids on epigenetic modification of class III histone deacetylase (HDAC)-sirtuins in glial cells.

Scientific reports

Sivalingam K,Doke M,Khan MA,Samikkannu T

Fri Oct 29 00:00:00 EDT 2021

SIRT4 regulates rat dental papilla cell differentiation by promoting mitochondrial functions.

The international journal of biochemistry & cell biology

Chen H,Kang J,Zhang F,Yan T,Fan W,He H,Huang F

Sat May 01 00:00:00 EDT 2021

SIRT4 regulates rat dental papilla cell differentiation by promoting mitochondrial functions.

The international journal of biochemistry & cell biology

Chen H,Kang J,Zhang F,Yan T,Fan W,He H,Huang F

Sat May 01 00:00:00 EDT 2021

SIRT4 regulates rat dental papilla cell differentiation by promoting mitochondrial functions.

The international journal of biochemistry & cell biology

Chen H,Kang J,Zhang F,Yan T,Fan W,He H,Huang F

Sat May 01 00:00:00 EDT 2021

miR-424-5p regulates cell proliferation and migration of esophageal squamous cell carcinoma by targeting SIRT4.

Journal of Cancer

Cui Y,Yang J,Bai Y,Zhang Y,Yao Y,Zheng T,Liu C,Wu F

Fri Nov 20 00:00:00 EST 2020

Ammonia inhibits energy metabolism in astrocytes in a rapid and glutamate dehydrogenase 2-dependent manner.

Disease models & mechanisms

Drews L,Zimmermann M,Westhoff P,Brilhaus D,Poss RE,Bergmann L,Wiek C,Brenneisen P,Piekorz RP,Mettler-Altmann T,Weber APM,Reichert AS

Wed Nov 04 00:00:00 EST 2020

Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.

Cell metabolism

Fu Y,Sun Y,Wang M,Hou Y,Huang W,Zhou D,Wang Z,Yang S,Tang W,Zhen J,Li Y,Wang X,Liu M,Zhang Y,Wang B,Liu G,Yu X,Sun J,Zhang C,Yi F

Tue Dec 01 00:00:00 EST 2020

SIRT4 enhances the sensitivity of ER-positive breast cancer to tamoxifen by inhibiting the IL-6/STAT3 signal pathway.

Cancer medicine

Xing J,Li J,Fu L,Gai J,Guan J,Li Q

Fri Nov 01 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: MGC130046; MGC130047; MGC57437; NAD-dependent ADP-ribosyltransferase sirtuin-4; NAD-dependent protein biotinylase sirtuin-4; NAD-dependent protein deacetylase sirtuin-4; NAD-dependent protein lipoamidase sirtuin-4, mitochondrial; Regulatory protein SIR2 homolog 4; sir2-like 4; SIR2-like protein 4; SIRT4; sirtuin type 4

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Gene Aliases: 4930596O17Rik; SIR2L4; SIRT4

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UniProt ID: (Human) Q9Y6E7, (Mouse) Q8R216

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Entrez Gene ID: (Pig) 100125349, (Human) 23409, (Mouse) 75387, (Rat) 304539

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Function(s)

protein ADP-ribosylation glutamine metabolic process cellular response to DNA damage stimulus negative regulation of cardiac muscle cell apoptotic process peptidyl-lysine deacetylation negative regulation of fatty acid oxidation negative regulation of insulin secretion positive regulation of lipid biosynthetic process tricarboxylic acid metabolic process negative regulation of protein processing involved in protein targeting to mitochondrion regulation of glutamine family amino acid metabolic process chromatin silencing cellular response to hypoxia regulation of pyruvate dehydrogenase activity

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SIRT4 Monoclonal Antibody (1D5A2)

SIRT4 Antibody (66543-1-IG) in WB

Product Details

66543-1-IG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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