Invitrogen

SIRT6 Monoclonal Antibody

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SIRT6 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of SIRT6 in HeLa cells fixed with 4% paraformaldehyde. Cells were probed with a SIRT6 monoclonal antibody (Product # MA5-24768) using a dilution of 1:100.

SIRT6 Antibody in Western Blot (WB) — Western blot analysis of SIRT6 in Jurkat, MCF7, HCT116, K562, COS7, C2C12, 3T3 and HeLa lysate. The membrane was probed with SIRT6 monoclonal antibody (Product # MA5-24768) with a dilution of 1:500.

Product Details

MA5-24768

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500	-
Immunocytochemistry (ICC/IF)	1:100	View 1 publication 1 publication
Immunoprecipitation (IP)	Assay-Dependent	-

Product Specifications
Species Reactivity	Human, Mouse, Non-human primate, Rat
Published species	Human
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	Purified recombinant human SIRT6 protein expressed in E.coli.
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS with 50% glycerol
Contains	0.09% sodium azide
Storage conditions	-20°C
RRID	AB_2717243

Target Information

Sirtuins regulate epigenetic gene silencing. SIRT6 (Mono-ADP-ribosyltransferase sirtuin-6), a mouse Sir2 homolog, is a chromatin-associated nuclear ADP-ribosyltransferase protein. It appears to be involved in DNA repair and may also play a role in human aging.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

SIRT6 Protects Against Liver Fibrosis by Deacetylation and Suppression of SMAD3 in Hepatic Stellate Cells.

Cellular and molecular gastroenterology and hepatology

Zhong X,Huang M,Kim HG,Zhang Y,Chowdhury K,Cai W,Saxena R,Schwabe RF,Liangpunsakul S,Dong XC

MA5-24768 was used in Immunocytochemistry to suggest that SIRT6 plays a critical role in the protection against NASH development and it may serve as a potential therapeutic target for NASH.

Fri Jul 09 00:00:00 EDT 2021

Bioinformatics

Protein Aliases: NAD-dependent deacetylase sirtuin-6; NAD-dependent protein deacetylase sirtuin-6; Regulatory protein SIR2 homolog 6; SIR2; SIR2 like 6; SIR2-like protein 6; sir2-related protein type 6; sirtuin type 6

Gene Aliases: 2810449N18Rik; AI043036; SIR2L6; SIRT6

UniProt ID: (Human) Q8N6T7, (Mouse) P59941

Entrez Gene ID: (Human) 51548, (Mouse) 50721, (Rat) 299638

Function(s)