OriGene

SIX1 Monoclonal Antibody (OTI1H4), TrueMAB™

View all (11) SIX1 antibodies

Questions & Answers

Questions & Answers

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SIX1 Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIX1 (RC203465, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-SIX1. Positive lysates LY401814 (100 µg) and LC401814 (20 µg) can be purchased separately from OriGene.

SIX1 Antibody in Western Blot (WB) — Western blot analysis of extracts (10 µg) from 4 different cell lines by using anti-SIX1 monoclonal antibody at 1:200 dilution.

Product Details

CF504057

Applications	Tested Dilution	Publications
Western Blot (WB)	1:2,000	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	OTI1H4
Immunogen	Human recombinant protein fragment corresponding to amino acids 1-284 of human SIX1 produced in E.coli. View antigen
Conjugate	Unconjugated
Form	lyophilized
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.3, with 8% trehalose
Contains	no preservative
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

The protein encoded by this gene is a homeobox protein that is similar to the Drosophila 'sine oculis' gene product. This gene is found in a cluster of related genes on chromosome 14 and is thought to be involved in limb development. Defects in this gene are a cause of autosomal dominant deafness type 23 (DFNA23) and branchiootic syndrome type 3 (BOS3).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Homeobox protein SIX1; Sine oculis homeobox homolog 1

Gene Aliases: BOS3; DFNA23; SIX1; TIP39

UniProt ID: (Human) Q15475

Entrez Gene ID: (Human) 6495

Molecular Function: homeodomain transcription factor

negative regulation of transcription from RNA polymerase II promoter ureteric bud development branching involved in ureteric bud morphogenesis organ induction kidney development outflow tract morphogenesis regulation of transcription, DNA-templated transcription from RNA polymerase II promoter apoptotic process pattern specification process skeletal muscle tissue development sensory perception of sound cell proliferation regulation of synaptic growth at neuromuscular junction regulation of skeletal muscle satellite cell proliferation facial nerve morphogenesis epithelial cell differentiation thyroid gland development olfactory placode formation regulation of protein localization protein localization to nucleus aorta morphogenesis inner ear morphogenesis middle ear morphogenesis negative regulation of neuron apoptotic process regulation of neuron differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter thymus development neuron fate specification generation of neurons embryonic cranial skeleton morphogenesis embryonic skeletal system morphogenesis inner ear development regulation of epithelial cell proliferation myoblast migration pharyngeal system development myotome development fungiform papilla morphogenesis trigeminal ganglion development otic vesicle development metanephric mesenchyme development regulation of branch elongation involved in ureteric bud branching positive regulation of ureteric bud formation mesonephric tubule formation ureter smooth muscle cell differentiation positive regulation of secondary heart field cardioblast proliferation cochlea morphogenesis positive regulation of branching involved in ureteric bud morphogenesis negative regulation of branching involved in ureteric bud morphogenesis positive regulation of mesenchymal cell proliferation involved in ureter development regulation of skeletal muscle cell differentiation

Process(es)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding sequence-specific DNA binding transcription regulatory region DNA binding