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SIX1 Antibody (CF504057) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIX1 (RC203465, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-SIX1. Positive lysates LY401814 (100 µg) and LC401814 (20 µg) can be purchased separately from OriGene.
Product Details
CF504057
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI1H4
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-284 of human SIX1 produced in E.coli.
Conjugate
Unconjugated
Form
lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
The protein encoded by this gene is a homeobox protein that is similar to the Drosophila 'sine oculis' gene product. This gene is found in a cluster of related genes on chromosome 14 and is thought to be involved in limb development. Defects in this gene are a cause of autosomal dominant deafness type 23 (DFNA23) and branchiootic syndrome type 3 (BOS3).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Homeobox protein SIX1; Sine oculis homeobox homolog 1
Gene Aliases: BOS3; DFNA23; SIX1; TIP39
UniProt ID: (Human) Q15475
Entrez Gene ID: (Human) 6495
Molecular Function:
homeodomain transcription factor
negative regulation of transcription from RNA polymerase II promoter
ureteric bud development
branching involved in ureteric bud morphogenesis
organ induction
kidney development
outflow tract morphogenesis
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
apoptotic process
pattern specification process
skeletal muscle tissue development
sensory perception of sound
cell proliferation
regulation of synaptic growth at neuromuscular junction
regulation of skeletal muscle satellite cell proliferation
facial nerve morphogenesis
epithelial cell differentiation
thyroid gland development
olfactory placode formation
regulation of protein localization
protein localization to nucleus
aorta morphogenesis
inner ear morphogenesis
middle ear morphogenesis
negative regulation of neuron apoptotic process
regulation of neuron differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
thymus development
neuron fate specification
generation of neurons
embryonic cranial skeleton morphogenesis
embryonic skeletal system morphogenesis
inner ear development
regulation of epithelial cell proliferation
myoblast migration
pharyngeal system development
myotome development
fungiform papilla morphogenesis
trigeminal ganglion development
otic vesicle development
metanephric mesenchyme development
regulation of branch elongation involved in ureteric bud branching
positive regulation of ureteric bud formation
mesonephric tubule formation
ureter smooth muscle cell differentiation
positive regulation of secondary heart field cardioblast proliferation
cochlea morphogenesis
positive regulation of branching involved in ureteric bud morphogenesis
negative regulation of branching involved in ureteric bud morphogenesis
positive regulation of mesenchymal cell proliferation involved in ureter development
regulation of skeletal muscle cell differentiation
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
sequence-specific DNA binding
transcription regulatory region DNA binding
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