Western blot analysis of SKP-1/p19A and Cullin-1 was performed by loading 10ug of whole cell lysates from DMSO, MLN4924 or MG132 treated U2OS cells onto a SDS PAGE gel. Proteins were transferred, blocked and endogenous SKP-1 (right panel) and Cullin-1 (left panel) were detected using a SKP-1 mouse monoclonal antibody (Prod# 32-3800) or CUL-1 mouse monoclonal antibody (Product# 32-2400) at dilutions of 1:1000.
|Tested species reactivity||Human|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant human SKP1 (p19) protein.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.1-1.0 ug/ml|
|Immunoprecipitation (IP)||5-10 ug/ml|
|Western Blot (WB)||1-3 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
SKP1 / p19A Antibody (1C10F4), Prod# 32-3800 specifically detects SKP1 in human cells. The antibody may also result in weak detection of 2 unknown bands at ~37kD.
This gene encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds directly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteosome. Specific F-box proteins recognize different target protein(s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
F-box protein FBXL16 binds PP2A-B55¿ and regulates differentiation of embryonic stem cells along the FLK1+ lineage.
32-3800 was used in western blot to study the interaction between F-box protein FBXL16 and PP2A-B55alpha and its effect on the differentiation of embryonic stem cells.
|Honarpour N,Rose CM,Brumbaugh J,Anderson J,Graham RL,Sweredoski MJ,Hess S,Coon JJ,Deshaies RJ||Molecular and cellular proteomics : MCP (13:780)||2014|
|Not Applicable||Not Cited||
Targeting the p27 E3 ligase SCF(Skp2) results in p27- and Skp2-mediated cell-cycle arrest and activation of autophagy.
32-3800 was used in western blot to study the result in p27- and Skp2-mediated cell-cycle arrest and activation of autophagy by targeting the p27 E3 ligase SCF(Skp2)
|Chen Q,Xie W,Kuhn DJ,Voorhees PM,Lopez-Girona A,Mendy D,Corral LG,Krenitsky VP,Xu W,Moutouh-de Parseval L,Webb DR,Mercurio F,Nakayama KI,Nakayama K,Orlowski RZ||Blood (111:4690)||2008|
|Not Applicable||Not Cited||
Hierarchical order of phosphorylation events commits Cdc25A to betaTrCP-dependent degradation.
32-3800 was used in western blot to study phosphorylation of Cdc25A
|Donzelli M,Busino L,Chiesa M,Ganoth D,Hershko A,Draetta GF||Cell cycle (Georgetown, Tex.) (3:469)||2004|
Degradation of Cdc25A by beta-TrCP during S phase and in response to DNA damage.
32-3800 was used in western blot to show that beta-TrCP targets phosphorylated Cdc25A for degradation by the Skp1/Cul1/F-box protein complex.
|Busino L,Donzelli M,Chiesa M,Guardavaccaro D,Ganoth D,Dorrello NV,Hershko A,Pagano M,Draetta GF||Nature (426:87)||2003|