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Immunofluorescent analysis of SLC2A5 using anti-SLC2A5 monoclonal antibody (Product# MA1-036) (shown in green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 1% Blocker BSA (Product #37525) for 15 minutes at room temperature. Cells were probed with a mouse monoclonal antibody recognizing SLC2A5 (Product# MA1-036), at a dilution of 1:100 for at least 1 hour at room temperature. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody (Product# 35503) at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Protein expressed in 293T cell transfected with human SLC2A5 expression vector.|
|Storage buffer||PBS with 1mg/ml BSA, 30% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-036 detects SLC2A5 in human and mouse samples and has successfully been used in Western blot, immunofluorescence, and immunohistochemistry applications. Note: This antibody requires overnight incubation in a Western blot for optimal performance. Do not use this antibody in a Fast Western blotting procedure.
Cytochalasin B-sensitive carrier. Seems to function primarily as a fructose transporter.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
facilitated glucose transporter member 5; fructose transporter; glucose transporter type 5; glucose transporter type 5, small intestine; glucose transporter-like protein 5; GLUT-5; GLUT5; RP5-963K15.1; small intestine; solute carrier family 2; solute carrier family 2 (facilitated glucose/fructose transporter), member 5; solute carrier family 2, facilitated glucose transporter member 5; testicular tissue protein Li 81
AI526984; GLUT-5; GLUT5; SLC2A5; Slc5a