Immunofluorescent analysis of SLC2A5 using anti-SLC2A5 monoclonal antibody (Product# MA1-036) (shown in green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 1% Blocker BSA (Product #37525) for 15 minutes at room temperature. Cells were probed with a mouse monoclonal antibody recognizing SLC2A5 (Product# MA1-036), at a dilution of 1:100 for at least 1 hour at room temperature. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody (Product# 35503) at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product# 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification. <br />
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Protein expressed in 293T cell transfected with human SLC2A5 expression vector.|
|Storage buffer||PBS with 1mg/ml BSA, 30% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-036X detects SLC2A5 in human and mouse samples and has successfully been used in western blot, immunofluorescence, and immunohistochemistry applications.
This antibody requires overnight incubation in a Western blot for optimal performance. Do not use this antibody in a Fast Western blotting procedure.
Cytochalasin B-sensitive carrier. Seems to function primarily as a fructose transporter.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.