OriGene

SLC2A5 Monoclonal Antibody (OTI14C8), TrueMAB™

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SLC2A5 Antibody in Immunocytochemistry (ICC/IF) — Anti-SLC2A5 mouse monoclonal antibody (TA500603) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SLC2A5(RC200418).

SLC2A5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded human endometrium tissue within the normal limits using anti-SLC2A5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500603)

SLC2A5 Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLC2A5 (RC200418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-SLC2A. Positive lysates LY401061 (100 µg) and LC401061 (20 µg) can be purchased separately from OriGene.

SLC2A5 Antibody in Flow Cytometry (Flow) — HEK293T cells transfected with either RC200418 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SLC2A5 antibody (TA500603), and then analyzed by flow cytometry.

SLC2A5 Antibody in Immunoprecipitation (IP) — Immunoprecipitation(IP) of SLC2A5 by using TrueMab monoclonal anti-SLC2A5 antibodies (Negative control: IP without adding anti-SLC2A5 antibody.). For each experiment, 500ul of tagged SLC2A5 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 µg of anti-SLC2A5 antibody and 20ul (0.1mg) of goat anti-mouse conj µgated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Product Details

CF500603

Applications	Tested Dilution	Publications
Western Blot (WB)	1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50	-
Immunocytochemistry (ICC/IF)	1:100	-
Flow Cytometry (Flow)	1:100	-
Immunoprecipitation (IP)	2 µg/500 µL	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	OTI14C8
Immunogen	Full length human recombinant protein of human SLC2A5 produced in HEK293T cell. View antigen
Conjugate	Unconjugated
Form	lyophilized
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.3, with 8% trehalose
Contains	no preservative
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

Cytochalasin B-sensitive carrier. Seems to function primarily as a fructose transporter.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Fructose transporter; Glucose transporter type 5, small intestine; glucose transporter-like protein 5; GLUT-5; RP5-963K15.1; solute carrier family 2 (facilitated glucose/fructose transporter), member 5; Solute carrier family 2, facilitated glucose transporter member 5; testicular tissue protein Li 81

Gene Aliases: GLUT-5; GLUT5; SLC2A5

UniProt ID: (Human) P22732

Entrez Gene ID: (Human) 6518

Function(s)

regulation of systemic arterial blood pressure mediated by a chemical signal carbohydrate metabolic process hexose transport response to fructose fructose transport glucose transport cellular response to fructose stimulus glucose transmembrane transport fructose import across plasma membrane

Process(es)

fructose transmembrane transporter activity glucose transmembrane transporter activity fructose binding