OriGene

SLC2A5 Monoclonal Antibody (OTI14C8), TrueMAB™

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SLC2A5 Antibody in Immunofluorescence (IF) — Anti-SLC2A5 mouse monoclonal antibody (TA500603) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SLC2A5(RC200418).

SLC2A5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded human endometrium tissue within the normal limits using anti-SLC2A5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500603)

SLC2A5 Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLC2A5 (RC200418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-SLC2A. Positive lysates LY401061 (100 µg) and LC401061 (20 µg) can be purchased separately from OriGene.

SLC2A5 Antibody in Flow Cytometry (Flow) — HEK293T cells transfected with either RC200418 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SLC2A5 antibody (TA500603), and then analyzed by flow cytometry.

SLC2A5 Antibody in Immunoprecipitation (IP) — Immunoprecipitation(IP) of SLC2A5 by using TrueMab monoclonal anti-SLC2A5 antibodies (Negative control: IP without adding anti-SLC2A5 antibody.). For each experiment, 500ul of tagged SLC2A5 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 µg of anti-SLC2A5 antibody and 20ul (0.1mg) of goat anti-mouse conj µgated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Product Details

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1:100

Immunocytochemistry (ICC)

1:100

Immunofluorescence (IF)

1:100

Immunohistochemistry (Paraffin) (IHC (P))

1:50

Immunoprecipitation (IP)

2 µg/500 µL

Western Blot (WB)

1:2000

Product Specifications

Species

Human

Expression System

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI14C8

Immunogen

Full length human recombinant protein of human SLC2A5 produced in HEK293T cell.

Conjugate

Unconjugated

Form

lyophilized

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Reconstitute with PBS (pH 7.3) and recommend to perform another round of desalting process using Product # 7KMWCO

Target Information

Cytochalasin B-sensitive carrier. Seems to function primarily as a fructose transporter.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Fructose transporter; Glucose transporter type 5, small intestine; glucose transporter-like protein 5; GLUT-5; RP5-963K15.1; solute carrier family 2 (facilitated glucose/fructose transporter), member 5; Solute carrier family 2, facilitated glucose transporter member 5; testicular tissue protein Li 81

Gene Aliases: GLUT-5; GLUT5; SLC2A5

UniProt ID: (Human) P22732

Entrez Gene ID: (Human) 6518

Function(s)

regulation of systemic arterial blood pressure mediated by a chemical signal carbohydrate metabolic process hexose transport response to fructose fructose transport glucose transport cellular response to fructose stimulus glucose transmembrane transport fructose import across plasma membrane

Process(es)

fructose transmembrane transporter activity glucose transmembrane transporter activity fructose binding