Western blot of SLC47A2/MATE-2 in human testis lysate was performed using 35ug protein in RIPA buffer. Lysates were probed with a SLC47A2/MATE-2 polyclonal antibody (Product# PA5-37989) at a dilution of 0.3ug/ml and incubated for 1 hour, followed by chemiluminescent detection.
|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Peptide with sequence SECHVDFFRTPEEAH, from the internal region near the C-terminus of SLC47A2|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.1-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
S47A2 belongs to a family of transporters involved in excretion of toxic electrolytes, both endogenous and exogenous, through urine and bile. This transporter family shares homology with the bacterial MATE (multidrug and toxin extrusion) protein family responsible for drug resistance. This gene is one of two members of the MATE transporter family located near each other on chromosome 17.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.