Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Product Details
MA1-46409
| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:500 | - |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:500 | - |
Immunohistochemistry (Frozen) (IHC (F)) |
1:500 | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human, Mouse, Non-human primate, Rat |
Host/Isotype |
Mouse / IgG1 |
Class |
Monoclonal |
Type |
Antibody |
Clone |
62-2E8 |
Immunogen |
Recombinant fusion protein from the C-terminal portion of the human CHT protein. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
Conc. Not Determined |
Storage buffer |
tissue culture supernatant with 50% glycerol |
Contains |
0.05% sodium azide |
Storage conditions |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_2189829 |
Product Specific Information
In Western blot a band can be seen at ~65 kDa representing CHT and a band can also be seen at ~45 kDa after deglycosylation with PNGase F.
Suggested positive control: antigen standard for SLC5A7 (transient overexpression lysate), rat striatum protein.
Target Information
Acetylcholine functions as a neurotransmitter in both the central and peripheral nervous systems of all vertebates, and is the principle neurotransmitter used at the neuromuscular junction. Presynaptic synthesis of acetylcholine requires a steady supply of choline, which is acquired by a plasma membrane high affinity choline transporter (CHT).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: CHT; hCHT; Hemicholinium-3-sensitive choline transporter; High affinity choline transporter 1; high affinity choline transporter; hemicholinium-3-sensitive choline transporter; MGC126299; MGC126300; solute carrier family 5 (choline transporter), member 7; solute carrier family 5 (sodium/choline cotransporter), member 7; Solute carrier family 5 member 7; solute carrier family 5, member 7
Gene Aliases: CHT; CHT1; HMN7A; SLC5A7
UniProt ID: (Human) Q9GZV3, (Mouse) Q8BGY9, (Rat) Q9JMD7
Entrez Gene ID: (Human) 60482, (Mouse) 63993, (Rat) 85426
Molecular Function:
transporter
sodium ion transport
neurotransmitter secretion
synaptic transmission, cholinergic
neuromuscular synaptic transmission
acetylcholine biosynthetic process
choline transport
transmembrane transport
transport
ion transport
organic cation transport
sodium ion transmembrane transport
neurotransmitter biosynthetic process
ammonium transmembrane transport
cation transmembrane transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support