Do not boil samples prior to Western Blot.
Suggested positive control: antigen standard for SLC9A8 (transient overexpression lysate), HeLa whole cell lysate..
Sodium-hydrogen exchangers (NHEs), such as SLC9A8, are integral transmembrane proteins that exchange extracellular Na+ for intracellular H+. NHEs have multiple functions, including intracellular pH homeostasis, cell volume regulation, and electroneutral NaCl absorption in epithelia (Xu et al., 2008).
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Protein Aliases: DKFZp686C03237; FLJ42500; MGC138418; Na(+)/H(+) exchanger 8; NHE-8; NHE-8 antibody; SLC9A8 antibody; Sodium/hydrogen exchanger 8; solute carrier family 9 (sodium/hydrogen exchanger), isoform 8; solute carrier family 9 (sodium/hydrogen exchanger), member 8; Solute carrier family 9 member 8; solute carrier family 9, subfamily A (NHE8, cation proton antiporter 8), member 8
Gene Aliases: 1200006P13Rik; 6430709P13Rik; AI182282; KIAA0939; NHE-8; NHE8; SLC9A8