|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified recombinant fragment of human SMAD3 expressed in E. Coli.|
|Contains||0.03% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1:200- 1:1000|
|Immunofluorescence (IF)||1:200 - 1:1000|
|Western Blot (WB)||1/500 - 1/2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
MA5-15663 targets SMAD3 in ICC, IF and WB applications and shows reactivity with Human and mouse samples.
The MA5-15663 immunogen is purified recombinant fragment of human SMAD3 expressed in E. Coli.
MA5-15663 detects SMAD3 which has a predicted molecular weight of approximately 48kDa.
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
c-Jun N terminal kinase modulates NOX-4 derived ROS production and myofibroblasts differentiation in human breast stromal cells.
MA5-15663 was used in western blot to examine the role of c-Jun N terminal kinase in myofibroblasts differentiation in human breast stromal cells
|Tobar N,Toyos M,Urra C,Méndez N,Arancibia R,Smith PC,Martínez J||BMC cancer (14:null)||2014|
Soluble MMP-14 produced by bone marrow-derived stromal cells sheds epithelial endoglin modulating the migratory properties of human breast cancer cells.
MA5-15663 was used in western blot to study the modulation of breast cancer cell migratory potential by endoglin shed from epithelial cells by soluble MMP14 from bone marrow-derived stromal cells
|Tobar N,Avalos MC,Méndez N,Smith PC,Bernabeu C,Quintanilla M,Martínez J||Carcinogenesis (35:1770)||2014|