|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified recombinant DPC4 protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
MA5-14297 targets SMAD4 in IF, IP, and WB applications and shows reactivity with Human samples.
The MA5-14297 immunogen is purified recombinant DPC4 protein.
DPC4 (also named SMAD4; small mothers against decapentaplegic deleted in pancreatic carcinoma). The family of SMAD molecules comprises essential mediators of transforming growth factor beta (TGF-beta) signaling. To date, seven members of this family have been identified, each of which plays a specific and separate role in mediating TGF-beta superfamily gene transcription. At least two different members, Smad2 and Smad4/DPC4, have been implicated in human cancer and appear to have tumor-suppressor functions whose inactivation may play an important role in the histogenesis of pancreatic and colorectal malignancy and possibly other human cancers as well.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Effect of DPC4 gene on invasion and metastasis of colorectal carcinoma cells.
MA5-14297 was used in immunohistochemistry and western blot to study the role of the DPC4 gene on invasion and metastasis of colorectal carcinoma cells
|Xiao DS,Wen JF,Li JH,Wang KS,Hu ZL,Zhou JH,Deng ZH,Liu Y||Acta biochimica et biophysica Sinica (38:883)||2006|