This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated. View Details
MA5-15682 targets SMAD4 in FACS, IF, IHC, and WB applications and shows reactivity with Human samples.
The MA5-15682 immunogen is purified recombinant fragment of human SMAD4 expressed in E. Coli.
MA5-15682 detects SMAD4 which has a predicted molecular weight of approximately 65kDa.
Smad4 is encoded by the Smad4 gene that is located on chromosome 18 in humans. Smad4 is also known as Smad family member 4, Sma- and Mad-related protein 4 or Mothers against decapentaplegic (MAD) homolog 4. Smad4 contains N-terminal MH1 (MAD homology 1) and C-terminal MH2 (MAD homology 2) globular domains that are involved in DNA binding and protein interactions respectively. Binding of the TGF-beta superfamily of ligands that includes Transforming Growth Factor -beta (TGF-beta) and bone morphogenetic protein (BMP) to its cognate receptor allows phosphorylation of Smad 1, 2, 3, 5 and 8 (R-Smad, Receptor Smad). This signals for heterotrimerization with Smad4 (co-Smad, co-mediator Smad) and translocation of the complex to the nucleus. Inhibitory or antagonistic Smad (I-Smad) that includes Smad 6 and 7, interact with activated R-Smads and attenuate the signaling pathway. Smad4 acts as a tumor suppressor protein by transcriptionally regulating its target genes such as Cyclin D1 (downregulation) and collagen (upregulation) that inhibit cell proliferation. Dephosphorylation regulates nuclear export and nucleocytoplasmic dynamics of Smads.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: deleted in pancreatic carcinoma locus 4; Deletion target in pancreatic carcinoma 4; Deletion target in pancreatic carcinoma 4 homolog; hSMAD4; MAD homolog 4; Mothers against decapentaplegic homolog 4; mothers against decapentaplegic, Drosophila, homolog of, 4; mothers against DPP homolog 4; SMAD; SMAD 4; SMAD family member 4; SMAD, mothers against DPP homolog 4
Gene Aliases: AW743858; D18Wsu70e; DPC4; JIP; MADH4; MYHRS; SMAD4