|Tested species reactivity||Fish, Human, Mouse, Zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide, conjugated to KLH, corresponding to residues A(294) L K L C S E L G L G G E F(307) of human SMARCB1 with N-terminally added Cystein|
|Purification||Antigen affinity chromatography|
|Contains||15mM sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1-2 ug/ml|
|Immunofluorescence (IF)||1-2 µg/ml|
|Western Blot (WB)||0.5-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-25450 detects SMARCB1 from human samples. PA1-25450 is expected to cross react with mouse (100% conserved) and zebrafish (100% conserved) due to sequence homology.
PA1-25450 has been successfully used in Western blot procedures.
The PA1-25450 immunogen is a synthetic peptide, conjugated to KLH, corresponding to residues A(294) L K L C S E L G L G G E F(307) of human SMARCB1 with N-terminally added Cysteine.
The protein encoded by this gene is part of a complex that relieves repressive chromatin structures, allowing the transcriptional machinery to access its targets more effectively. The encoded nuclear protein may also bind to and enhance the DNA joining activity of HIV-1 integrase. This gene has been found to be a tumor suppressor, and mutations in it have been associated with malignant rhabdoid tumors. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.