Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Structural Maintenance of Chromosomes (SMC) family proteins play critical roles in various nuclear events that require structural changes of chromosomes, including mitotic chromosome organization, DNA recombination and repair and global transcriptional repression. The chromo proteins are conserved in eukaryotes lead to mitotic chromosome segregatior defects, suggesting a critical function of SMC family proteins in mitotic chromosome dynamics. SMC1 and SMC3 form a heterodimeric complex required for metaphase progression in mitotic cells. Specifically this SMC1/SMC3 complex is responsible for sister chromatid cohesion during metaphase. A number of cellular factors interact with hSMC1/hSMC3 during cell cycle. The major population of hSMC1/hSMC3 is in a compex with hRAD21 forming the human cohesion complex. Human cohesion associates with chromosomes which peaks at S phase and dissociates from chromosomes during G2/M transition. In addition, a subpopulation of hSMC1/hSMC3 associates tightly with nuclear matrix and centrosomes during interphase. A subset of hSMC1/hSMC3 is localized to spindle poles, spindles, and kinetochores during mitosis when cohesin is in the cytoplasm. hSMC1/hSMC3 is required for spindle aster formation in vitro and reacts with nuclear mitotic apparatus protein in vivo.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Acute myeloid leukemia 2 protein; acute myeloid leukemia gene 2; CBF-alpha-3; Chromosome segregation protein SmcB; core binding factor alpha 3; Core-binding factor subunit alpha-3; core-binding factor, runt domain, alpha subunit 3; DXhXs423e; mitosis-specific chromosome segregation protein like protein beta; Oncogene AML-2; PEA2 alpha C; PEA2-alpha C; PEBP2 alpha C; PEBP2-alpha C; Polyomavirus enhancer-binding protein 2 alpha C subunit; runt domain, alpha subunit 3; Runt related transcription factor 3; Runt-related transcription factor 3; Sb1.8; segregation of mitotic chromosomes 1; segregation of mitotic chromosomes b; segregation of mitotic chromosomes-like 1; SL3-3 enhancer factor 1 alpha C subunit; SL3/AKV core-binding factor alpha C subunit; SMC; SMC (segregation of mitotic chromosomes 1)-like 1; SMC protein 1A; SMC protein 1B; SMC-1-alpha; SMC-1-beta; SMC-like 1; SMC-protein; SMC1 (structural maintenance of chromosomes 1, yeast)-like 1; SMC1 structural maintenance of chromosomes 1-like 2; structural maintenace of chromosomes 1B; structural maintenance of chromosomes 1 like 1; Structural maintenance of chromosomes protein 1A; Structural maintenance of chromosomes protein 1B; transcription factor AML2; transcription factor AML2/CBFA3
Gene Aliases: 5830426I24Rik; AML2; CBFA3; CDLS2; DXS423E; KIAA0178; mKIAA0178; PEBP2A3; PEBP2aC; RUNX3; SB1.8; SMC-1A; SMC-1B; SMC1; SMC1A; SMC1alpha; SMC1B; SMC1BETA; SMC1L1; SMC1L2; SMCB